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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Putative arginine/ornithine antiporter (ydgI), partial CSB-YP364481EGX
CSB-EP364481EGX
CSB-BP364481EGX
CSB-MP364481EGX
CSB-EP364481EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Probable cadaverine/lysine antiporter (cadB), partial CSB-YP364482ENV
CSB-EP364482ENV
CSB-BP364482ENV
CSB-MP364482ENV
CSB-EP364482ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable cadaverine/lysine antiporter (cadB), partial CSB-YP364483EOD
CSB-EP364483EOD
CSB-BP364483EOD
CSB-MP364483EOD
CSB-EP364483EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putrescine-ornithine antiporter (potE), partial CSB-YP364484EGX
CSB-EP364484EGX
CSB-BP364484EGX
CSB-MP364484EGX
CSB-EP364484EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabinose import ATP-binding protein AraG (araG) CSB-YP364485EGX
CSB-EP364485EGX
CSB-BP364485EGX
CSB-MP364485EGX
CSB-EP364485EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Arginine transport ATP-binding protein ArtP (artP) CSB-YP364486ENV
CSB-EP364486ENV
CSB-BP364486ENV
CSB-MP364486ENV
CSB-EP364486ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arginine transport ATP-binding protein ArtP (artP) CSB-YP364487EOD
CSB-EP364487EOD
CSB-BP364487EOD
CSB-MP364487EOD
CSB-EP364487EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Dipeptide transport ATP-binding protein DppD (dppD) CSB-YP364488ENV
CSB-EP364488ENV
CSB-BP364488ENV
CSB-MP364488ENV
CSB-EP364488ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dipeptide transport ATP-binding protein DppD (dppD) CSB-YP364489SZB
CSB-EP364489SZB
CSB-BP364489SZB
CSB-MP364489SZB
CSB-EP364489SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutamate/aspartate transport ATP-binding protein GltL (gltL) CSB-YP364490EOD
CSB-EP364490EOD
CSB-BP364490EOD
CSB-MP364490EOD
CSB-EP364490EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Multidrug resistance-like ATP-binding protein MdlB (mdlB), partial CSB-YP364491EGX1
CSB-EP364491EGX1
CSB-BP364491EGX1
CSB-MP364491EGX1
CSB-EP364491EGX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Galactose/methyl galactoside import ATP-binding protein MglA (mglA) CSB-YP364492ENV
CSB-EP364492ENV
CSB-BP364492ENV
CSB-MP364492ENV
CSB-EP364492ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Phosphate import ATP-binding protein PstB (pstB) CSB-YP364493ENV
CSB-EP364493ENV
CSB-BP364493ENV
CSB-MP364493ENV
CSB-EP364493ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphate import ATP-binding protein PstB (pstB) CSB-YP364494EOD
CSB-EP364494EOD
CSB-BP364494EOD
CSB-MP364494EOD
CSB-EP364494EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Peptide transport system ATP-binding protein SapD (sapD) CSB-YP364495ENV
CSB-EP364495ENV
CSB-BP364495ENV
CSB-MP364495ENV
CSB-EP364495ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Peptide transport system ATP-binding protein SapD (sapD) CSB-YP364496EOD
CSB-EP364496EOD
CSB-BP364496EOD
CSB-MP364496EOD
CSB-EP364496EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Peptide transport system ATP-binding protein SapF (sapF) CSB-YP364497ENV
CSB-EP364497ENV
CSB-BP364497ENV
CSB-MP364497ENV
CSB-EP364497ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Peptide transport system ATP-binding protein SapF (sapF) CSB-YP364498SZB
CSB-EP364498SZB
CSB-BP364498SZB
CSB-MP364498SZB
CSB-EP364498SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliphatic sulfonates import ATP-binding protein SsuB (ssuB) CSB-YP364499EOD
CSB-EP364499EOD
CSB-BP364499EOD
CSB-MP364499EOD
CSB-EP364499EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP-dependent zinc metalloprotease FtsH (ftsH), partial CSB-YP364500SZB
CSB-EP364500SZB
CSB-BP364500SZB
CSB-MP364500SZB
CSB-EP364500SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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