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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Dihydroneopterin triphosphate pyrophosphatase (nudB) CSB-YP365288SZB
CSB-EP365288SZB
CSB-BP365288SZB
CSB-MP365288SZB
CSB-EP365288SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Nucleoside permease nupC (nupC), partial CSB-YP365289ENV
CSB-EP365289ENV
CSB-BP365289ENV
CSB-MP365289ENV
CSB-EP365289ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Nucleoside permease nupG (nupG), partial CSB-YP365290ENV
CSB-EP365290ENV
CSB-BP365290ENV
CSB-MP365290ENV
CSB-EP365290ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Transcription elongation protein nusA (nusA) CSB-YP365291ENV
CSB-EP365291ENV
CSB-BP365291ENV
CSB-MP365291ENV
CSB-EP365291ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Transcription elongation protein nusA (nusA) CSB-YP365292SZB
CSB-EP365292SZB
CSB-BP365292SZB
CSB-MP365292SZB
CSB-EP365292SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Transcription termination/antitermination protein NusG (nusG) CSB-YP365293ENV
CSB-EP365293ENV
CSB-BP365293ENV
CSB-MP365293ENV
CSB-EP365293ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Transcription antitermination protein NusG (nusG) CSB-YP365294SZB
CSB-EP365294SZB
CSB-BP365294SZB
CSB-MP365294SZB
CSB-EP365294SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 2-oxoglutarate dehydrogenase E1 component (sucA), partial CSB-YP365295EGX
CSB-EP365295EGX
CSB-BP365295EGX
CSB-MP365295EGX
CSB-EP365295EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Pyruvate dehydrogenase E1 component (aceE), partial CSB-YP365296ENV
CSB-EP365296ENV
CSB-BP365296ENV
CSB-MP365296ENV
CSB-EP365296ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Oligopeptide transport system permease protein oppB (oppB), partial CSB-YP365297ENV1
CSB-EP365297ENV1
CSB-BP365297ENV1
CSB-MP365297ENV1
CSB-EP365297ENV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligopeptide transport system permease protein oppB (oppB), partial CSB-YP365298EOD1
CSB-EP365298EOD1
CSB-BP365298EOD1
CSB-MP365298EOD1
CSB-EP365298EOD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Oligopeptide transport system permease protein oppC (oppC), partial CSB-YP365299ENV1
CSB-EP365299ENV1
CSB-BP365299ENV1
CSB-MP365299ENV1
CSB-EP365299ENV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Osmotically-inducible protein Y (osmY) CSB-YP365300ENV
CSB-EP365300ENV
CSB-BP365300ENV
CSB-MP365300ENV
CSB-EP365300ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable oxalyl-CoA decarboxylase (oxc) CSB-YP365301EOD
CSB-EP365301EOD
CSB-BP365301EOD
CSB-MP365301EOD
CSB-EP365301EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA topoisomerase 4 subunit A (parC) CSB-YP365302EOD
CSB-EP365302EOD
CSB-BP365302EOD
CSB-MP365302EOD
CSB-EP365302EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA topoisomerase 4 subunit A (parC), partial CSB-YP365303SZB
CSB-EP365303SZB
CSB-BP365303SZB
CSB-MP365303SZB
CSB-EP365303SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant D-alanyl-D-alanine endopeptidase (pbpG) CSB-YP365304SZB
CSB-EP365304SZB
CSB-BP365304SZB
CSB-MP365304SZB
CSB-EP365304SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative permease perM (perM), partial CSB-YP365306EOD1
CSB-EP365306EOD1
CSB-BP365306EOD1
CSB-MP365306EOD1
CSB-EP365306EOD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein phnA (phnA) CSB-YP365307EGX
CSB-EP365307EGX
CSB-BP365307EGX
CSB-MP365307EGX
CSB-EP365307EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein phnA (phnA) CSB-YP365308SZB
CSB-EP365308SZB
CSB-BP365308SZB
CSB-MP365308SZB
CSB-EP365308SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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