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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Streptomycin 3'-adenylyltransferase (aadA) CSB-YP365389SZB
CSB-EP365389SZB
CSB-BP365389SZB
CSB-MP365389SZB
CSB-EP365389SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein umuD (umuD) CSB-YP365390EOD
CSB-EP365390EOD
CSB-BP365390EOD
CSB-MP365390EOD
CSB-EP365390EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Probable protease sohB (sohB), partial CSB-YP365391ENV
CSB-EP365391ENV
CSB-BP365391ENV
CSB-MP365391ENV
CSB-EP365391ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant N5-carboxyaminoimidazole ribonucleotide mutase (purE) CSB-YP365392EOD
CSB-EP365392EOD
CSB-BP365392EOD
CSB-MP365392EOD
CSB-EP365392EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant GTP pyrophosphokinase (relA) CSB-YP365393EGX
CSB-EP365393EGX
CSB-BP365393EGX
CSB-MP365393EGX
CSB-EP365393EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant GTP pyrophosphokinase (relA), partial CSB-YP365394SZB
CSB-EP365394SZB
CSB-BP365394SZB
CSB-MP365394SZB
CSB-EP365394SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein yibN (yibN) CSB-YP365396ENV
CSB-EP365396ENV
CSB-BP365396ENV
CSB-MP365396ENV
CSB-EP365396ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein yibN (yibN) CSB-YP365397EOD
CSB-EP365397EOD
CSB-BP365397EOD
CSB-MP365397EOD
CSB-EP365397EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Transcription termination factor Rho (rho) CSB-YP365398EGX
CSB-EP365398EGX
CSB-BP365398EGX
CSB-MP365398EGX
CSB-EP365398EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Transcription termination factor Rho (rho) CSB-YP365399SZB
CSB-EP365399SZB
CSB-BP365399SZB
CSB-MP365399SZB
CSB-EP365399SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Homoserine/homoserine lactone efflux protein (rhtB), partial CSB-YP365400EGX1
CSB-EP365400EGX1
CSB-BP365400EGX1
CSB-MP365400EGX1
CSB-EP365400EGX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Homoserine/homoserine lactone efflux protein (rhtB), partial CSB-YP365401SZB1
CSB-EP365401SZB1
CSB-BP365401SZB1
CSB-MP365401SZB1
CSB-EP365401SZB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Camphor 5-monooxygenase (camC) CSB-YP365402FFZ
CSB-EP365402FFZ
CSB-BP365402FFZ
CSB-MP365402FFZ
CSB-EP365402FFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ferredoxin CSB-YP365403CMA
CSB-EP365403CMA
CSB-BP365403CMA
CSB-MP365403CMA
CSB-EP365403CMA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ferredoxin CSB-YP365404CLN
CSB-EP365404CLN
CSB-BP365404CLN
CSB-MP365404CLN
CSB-EP365404CLN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Moorella thermoacetica Ferredoxin CSB-YP365405MUF
CSB-EP365405MUF
CSB-BP365405MUF
CSB-MP365405MUF
CSB-EP365405MUF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Allochromatium vinosum Ferredoxin (fdx) CSB-YP365406AZP
CSB-EP365406AZP
CSB-BP365406AZP
CSB-MP365406AZP
CSB-EP365406AZP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus stearothermophilus Ferredoxin (fer) CSB-YP365407GFM
CSB-EP365407GFM
CSB-BP365407GFM
CSB-MP365407GFM
CSB-EP365407GFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus acidocaldarius Zinc-containing ferredoxin (zfx) CSB-YP365408FOZ
CSB-EP365408FOZ
CSB-BP365408FOZ
CSB-MP365408FOZ
CSB-EP365408FOZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arctium lappa Ferredoxin CSB-YP365409ANQ
CSB-EP365409ANQ
CSB-BP365409ANQ
CSB-MP365409ANQ
CSB-EP365409ANQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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