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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Listeria welshimeri serovar 6b Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP366806LLT
CSB-EP366806LLT
CSB-BP366806LLT
CSB-MP366806LLT
CSB-EP366806LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b Segregation and condensation protein B (scpB) CSB-YP366807LLT
CSB-EP366807LLT
CSB-BP366807LLT
CSB-MP366807LLT
CSB-EP366807LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b Phosphopentomutase (deoB) CSB-YP366808LLT
CSB-EP366808LLT
CSB-BP366808LLT
CSB-MP366808LLT
CSB-EP366808LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b Dihydroxy-acid dehydratase (ilvD), partial CSB-YP366809LLT
CSB-EP366809LLT
CSB-BP366809LLT
CSB-MP366809LLT
CSB-EP366809LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b Ketol-acid reductoisomerase (ilvC) CSB-YP366810LLT
CSB-EP366810LLT
CSB-BP366810LLT
CSB-MP366810LLT
CSB-EP366810LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP366811LLT
CSB-EP366811LLT
CSB-BP366811LLT
CSB-MP366811LLT
CSB-EP366811LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP366812LLT
CSB-EP366812LLT
CSB-BP366812LLT
CSB-MP366812LLT
CSB-EP366812LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b Diaminopimelate epimerase (dapF) CSB-YP366813LLT
CSB-EP366813LLT
CSB-BP366813LLT
CSB-MP366813LLT
CSB-EP366813LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b Cell division protein SepF (sepF) CSB-YP366814LLT
CSB-EP366814LLT
CSB-BP366814LLT
CSB-MP366814LLT
CSB-EP366814LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP366815LLT
CSB-EP366815LLT
CSB-BP366815LLT
CSB-MP366815LLT
CSB-EP366815LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b 50S ribosomal protein L32 2 (rpmF2) CSB-YP366816LLT
CSB-EP366816LLT
CSB-BP366816LLT
CSB-MP366816LLT
CSB-EP366816LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b Phosphopantetheine adenylyltransferase (coaD) CSB-YP366817LLT
CSB-EP366817LLT
CSB-BP366817LLT
CSB-MP366817LLT
CSB-EP366817LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b Large-conductance mechanosensitive channel (mscL), partial CSB-YP366818LLT1
CSB-EP366818LLT1
CSB-BP366818LLT1
CSB-MP366818LLT1
CSB-EP366818LLT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b 10 kDa chaperonin (groS) CSB-YP366819LLT
CSB-EP366819LLT
CSB-BP366819LLT
CSB-MP366819LLT
CSB-EP366819LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b Redox-sensing transcriptional repressor rex (rex) CSB-YP366820LLT
CSB-EP366820LLT
CSB-BP366820LLT
CSB-MP366820LLT
CSB-EP366820LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b Glutamine amidotransferase subunit pdxT (pdxT) CSB-YP366821LLT
CSB-EP366821LLT
CSB-BP366821LLT
CSB-MP366821LLT
CSB-EP366821LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b Phosphoglucosamine mutase (glmM) CSB-YP366822LLT
CSB-EP366822LLT
CSB-BP366822LLT
CSB-MP366822LLT
CSB-EP366822LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b 3-oxoacyl-[acyl-carrier-protein] synthase 3 (fabH) CSB-YP366823LLT
CSB-EP366823LLT
CSB-BP366823LLT
CSB-MP366823LLT
CSB-EP366823LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b UPF0342 protein lwe2240 (lwe2240) CSB-YP366824LLT
CSB-EP366824LLT
CSB-BP366824LLT
CSB-MP366824LLT
CSB-EP366824LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b UPF0344 protein lwe2280 (lwe2280), partial CSB-YP366825LLT1
CSB-EP366825LLT1
CSB-BP366825LLT1
CSB-MP366825LLT1
CSB-EP366825LLT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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