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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Listeria welshimeri serovar 6b Energy-coupling factor transporter ATP-binding protein EcfA 2 (ecfA2) CSB-YP366506LLT
CSB-EP366506LLT
CSB-BP366506LLT
CSB-MP366506LLT
CSB-EP366506LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b 50S ribosomal protein L17 (rplQ) CSB-YP366507LLT
CSB-EP366507LLT
CSB-BP366507LLT
CSB-MP366507LLT
CSB-EP366507LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP366508LLT
CSB-EP366508LLT
CSB-BP366508LLT
CSB-MP366508LLT
CSB-EP366508LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b 30S ribosomal protein S13 (rpsM) CSB-YP366509LLT
CSB-EP366509LLT
CSB-BP366509LLT
CSB-MP366509LLT
CSB-EP366509LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b Translation initiation factor IF-1 (infA) CSB-YP366510LLT
CSB-EP366510LLT
CSB-BP366510LLT
CSB-MP366510LLT
CSB-EP366510LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b 50S ribosomal protein L15 (rplO) CSB-YP366511LLT
CSB-EP366511LLT
CSB-BP366511LLT
CSB-MP366511LLT
CSB-EP366511LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b 30S ribosomal protein S5 (rpsE) CSB-YP366512LLT
CSB-EP366512LLT
CSB-BP366512LLT
CSB-MP366512LLT
CSB-EP366512LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b 50S ribosomal protein L6 (rplF) CSB-YP366513LLT
CSB-EP366513LLT
CSB-BP366513LLT
CSB-MP366513LLT
CSB-EP366513LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b 30S ribosomal protein S14 type Z (rpsZ) CSB-YP366514LLT
CSB-EP366514LLT
CSB-BP366514LLT
CSB-MP366514LLT
CSB-EP366514LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b 50S ribosomal protein L24 (rplX) CSB-YP366515LLT
CSB-EP366515LLT
CSB-BP366515LLT
CSB-MP366515LLT
CSB-EP366515LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b 30S ribosomal protein S17 (rpsQ) CSB-YP366516LLT
CSB-EP366516LLT
CSB-BP366516LLT
CSB-MP366516LLT
CSB-EP366516LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b 30S ribosomal protein S3 (rpsC) CSB-YP366517LLT
CSB-EP366517LLT
CSB-BP366517LLT
CSB-MP366517LLT
CSB-EP366517LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b 50S ribosomal protein L22 (rplV) CSB-YP366518LLT
CSB-EP366518LLT
CSB-BP366518LLT
CSB-MP366518LLT
CSB-EP366518LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b 50S ribosomal protein L2 (rplB) CSB-YP366519LLT
CSB-EP366519LLT
CSB-BP366519LLT
CSB-MP366519LLT
CSB-EP366519LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b 50S ribosomal protein L23 (rplW) CSB-YP366520LLT
CSB-EP366520LLT
CSB-BP366520LLT
CSB-MP366520LLT
CSB-EP366520LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b 50S ribosomal protein L3 (rplC) CSB-YP366521LLT
CSB-EP366521LLT
CSB-BP366521LLT
CSB-MP366521LLT
CSB-EP366521LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b 30S ribosomal protein S7 (rpsG) CSB-YP366522LLT
CSB-EP366522LLT
CSB-BP366522LLT
CSB-MP366522LLT
CSB-EP366522LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b Potassium-transporting ATPase B chain (kdpB), partial CSB-YP366523LLT1
CSB-EP366523LLT1
CSB-BP366523LLT1
CSB-MP366523LLT1
CSB-EP366523LLT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b Recombination protein RecR (recR) CSB-YP366524LLT
CSB-EP366524LLT
CSB-BP366524LLT
CSB-MP366524LLT
CSB-EP366524LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria welshimeri serovar 6b UPF0133 protein lwe2654 (lwe2654) CSB-YP366525LLT
CSB-EP366525LLT
CSB-BP366525LLT
CSB-MP366525LLT
CSB-EP366525LLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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