Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Burkholderia cenocepacia 4-hydroxyproline epimerase (Bcen2424_4140) CSB-YP366546BPO
CSB-EP366546BPO
CSB-BP366546BPO
CSB-MP366546BPO
CSB-EP366546BPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia Translation initiation factor IF-1 2 (infA2) CSB-YP366547BPO
CSB-EP366547BPO
CSB-BP366547BPO
CSB-MP366547BPO
CSB-EP366547BPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia Aliphatic amidase (amiE) CSB-YP366548BPO
CSB-EP366548BPO
CSB-BP366548BPO
CSB-MP366548BPO
CSB-EP366548BPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia Ribose import ATP-binding protein RbsA 2 (rbsA2), partial CSB-YP366549BPO
CSB-EP366549BPO
CSB-BP366549BPO
CSB-MP366549BPO
CSB-EP366549BPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia Putative ribose/galactose/methyl galactoside import ATP-binding protein 2 (Bcen2424_5039), partial CSB-YP366550BPO
CSB-EP366550BPO
CSB-BP366550BPO
CSB-MP366550BPO
CSB-EP366550BPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia HTH-type transcriptional regulator BetI (betI) CSB-YP366551BPO
CSB-EP366551BPO
CSB-BP366551BPO
CSB-MP366551BPO
CSB-EP366551BPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia Catalase-peroxidase 2 (katG2), partial CSB-YP366552BPO
CSB-EP366552BPO
CSB-BP366552BPO
CSB-MP366552BPO
CSB-EP366552BPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE), partial CSB-YP366553BPO
CSB-EP366553BPO
CSB-BP366553BPO
CSB-MP366553BPO
CSB-EP366553BPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia Methionine import ATP-binding protein MetN 2 (metN2) CSB-YP366554BPO
CSB-EP366554BPO
CSB-BP366554BPO
CSB-MP366554BPO
CSB-EP366554BPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia Ureidoglycolate lyase (Bcen2424_5521) CSB-YP366555BPO
CSB-EP366555BPO
CSB-BP366555BPO
CSB-MP366555BPO
CSB-EP366555BPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia UPF0502 protein Bcen2424_5610 (Bcen2424_5610) CSB-YP366556BPO
CSB-EP366556BPO
CSB-BP366556BPO
CSB-MP366556BPO
CSB-EP366556BPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia Arabinose import ATP-binding protein AraG 2 (araG2), partial CSB-YP366557BPO
CSB-EP366557BPO
CSB-BP366557BPO
CSB-MP366557BPO
CSB-EP366557BPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP366558BPO
CSB-EP366558BPO
CSB-BP366558BPO
CSB-MP366558BPO
CSB-EP366558BPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia Probable acetoacetate decarboxylase (adc) CSB-YP366559BPO
CSB-EP366559BPO
CSB-BP366559BPO
CSB-MP366559BPO
CSB-EP366559BPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanosaeta thermophila RNA 3'-terminal phosphate cyclase (rtcA) CSB-YP366560MNW
CSB-EP366560MNW
CSB-BP366560MNW
CSB-MP366560MNW
CSB-EP366560MNW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanosaeta thermophila DNA-directed RNA polymerase subunit H (rpoH) CSB-YP366561MNW
CSB-EP366561MNW
CSB-BP366561MNW
CSB-MP366561MNW
CSB-EP366561MNW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanosaeta thermophila 50S ribosomal protein L30e (rpl30e) CSB-YP366562MNW
CSB-EP366562MNW
CSB-BP366562MNW
CSB-MP366562MNW
CSB-EP366562MNW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanosaeta thermophila Acylphosphatase (acyP) CSB-YP366563MNW
CSB-EP366563MNW
CSB-BP366563MNW
CSB-MP366563MNW
CSB-EP366563MNW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanosaeta thermophila Proteasome subunit beta (psmB) CSB-YP366564MNW
CSB-EP366564MNW
CSB-BP366564MNW
CSB-MP366564MNW
CSB-EP366564MNW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanosaeta thermophila D-tyrosyl-tRNA (Tyr) deacylase CSB-YP366565MNW
CSB-EP366565MNW
CSB-BP366565MNW
CSB-MP366565MNW
CSB-EP366565MNW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>