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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Francisella tularensis subsp. novicida Glutamyl-tRNA (Gln) amidotransferase subunit A CSB-YP367846FDW
CSB-EP367846FDW
CSB-BP367846FDW
CSB-MP367846FDW
CSB-EP367846FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. novicida Potassium-transporting ATPase A chain (kdpA), partial CSB-YP367847FDW
CSB-EP367847FDW
CSB-BP367847FDW
CSB-MP367847FDW
CSB-EP367847FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. novicida Phosphoribosylglycinamide formyltransferase 2 (purT) CSB-YP367848FDW
CSB-EP367848FDW
CSB-BP367848FDW
CSB-MP367848FDW
CSB-EP367848FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium avium DNA replication and repair protein recF (recF) CSB-YP367849MOI
CSB-EP367849MOI
CSB-BP367849MOI
CSB-MP367849MOI
CSB-EP367849MOI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium avium Leucine--tRNA ligase (leuS), partial CSB-YP367850MOI
CSB-EP367850MOI
CSB-BP367850MOI
CSB-MP367850MOI
CSB-EP367850MOI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium avium 50S ribosomal protein L9 (rplI) CSB-YP367851MOI
CSB-EP367851MOI
CSB-BP367851MOI
CSB-MP367851MOI
CSB-EP367851MOI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium avium Prephenate dehydratase (pheA) CSB-YP367852MOI
CSB-EP367852MOI
CSB-BP367852MOI
CSB-MP367852MOI
CSB-EP367852MOI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium avium Serine--tRNA ligase (serS) CSB-YP367853MOI
CSB-EP367853MOI
CSB-BP367853MOI
CSB-MP367853MOI
CSB-EP367853MOI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium avium DNA integrity scanning protein DisA (disA) CSB-YP367854MOI
CSB-EP367854MOI
CSB-BP367854MOI
CSB-MP367854MOI
CSB-EP367854MOI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium avium Cysteine--tRNA ligase (cysS) CSB-YP367855MOI
CSB-EP367855MOI
CSB-BP367855MOI
CSB-MP367855MOI
CSB-EP367855MOI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium avium UPF0271 protein MAV_0580 (MAV_0580) CSB-YP367856MOI
CSB-EP367856MOI
CSB-BP367856MOI
CSB-MP367856MOI
CSB-EP367856MOI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium avium Phosphoribosylformylglycinamidine synthase 2 (purL), partial CSB-YP367857MOI
CSB-EP367857MOI
CSB-BP367857MOI
CSB-MP367857MOI
CSB-EP367857MOI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium avium Putative S-adenosyl-L-methionine-dependent methyltransferase MAV_0778 (MAV_0778) CSB-YP367858MOI
CSB-EP367858MOI
CSB-BP367858MOI
CSB-MP367858MOI
CSB-EP367858MOI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium avium Putative phosphoserine aminotransferase (serC) CSB-YP367859MOI
CSB-EP367859MOI
CSB-BP367859MOI
CSB-MP367859MOI
CSB-EP367859MOI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium avium Putative S-adenosyl-L-methionine-dependent methyltransferase MAV_1058 (MAV_1058) CSB-YP367860MOI
CSB-EP367860MOI
CSB-BP367860MOI
CSB-MP367860MOI
CSB-EP367860MOI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium avium 50S ribosomal protein L32 (rpmF) CSB-YP367861MOI
CSB-EP367861MOI
CSB-BP367861MOI
CSB-MP367861MOI
CSB-EP367861MOI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium avium Signal transduction histidine-protein kinase/phosphatase mprB (mprB), partial CSB-YP367862MOI
CSB-EP367862MOI
CSB-BP367862MOI
CSB-MP367862MOI
CSB-EP367862MOI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium avium Enolase (eno) CSB-YP367863MOI
CSB-EP367863MOI
CSB-BP367863MOI
CSB-MP367863MOI
CSB-EP367863MOI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium avium Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP367864MOI
CSB-EP367864MOI
CSB-BP367864MOI
CSB-MP367864MOI
CSB-EP367864MOI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium avium 50S ribosomal protein L31 (rpmE) CSB-YP367865MOI
CSB-EP367865MOI
CSB-BP367865MOI
CSB-MP367865MOI
CSB-EP367865MOI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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