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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Burkholderia cenocepacia UPF0042 nucleotide-binding protein Bcen2424_2795 (Bcen2424_2795) CSB-YP368406BPO
CSB-EP368406BPO
CSB-BP368406BPO
CSB-MP368406BPO
CSB-EP368406BPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia Phosphopantetheine adenylyltransferase (coaD) CSB-YP368407BPO
CSB-EP368407BPO
CSB-BP368407BPO
CSB-MP368407BPO
CSB-EP368407BPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia Protein-export protein secB (secB) CSB-YP368408BPO
CSB-EP368408BPO
CSB-BP368408BPO
CSB-MP368408BPO
CSB-EP368408BPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia Octanoyltransferase (lipB) CSB-YP368409BPO
CSB-EP368409BPO
CSB-BP368409BPO
CSB-MP368409BPO
CSB-EP368409BPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia Biotin synthase (bioB) CSB-YP368410BPO
CSB-EP368410BPO
CSB-BP368410BPO
CSB-MP368410BPO
CSB-EP368410BPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia Multifunctional CCA protein (cca) CSB-YP368411BPO
CSB-EP368411BPO
CSB-BP368411BPO
CSB-MP368411BPO
CSB-EP368411BPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP368412BPO
CSB-EP368412BPO
CSB-BP368412BPO
CSB-MP368412BPO
CSB-EP368412BPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP368413BPO
CSB-EP368413BPO
CSB-BP368413BPO
CSB-MP368413BPO
CSB-EP368413BPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia Protease HtpX homolog (htpX), partial CSB-YP368414BPO1
CSB-EP368414BPO1
CSB-BP368414BPO1
CSB-MP368414BPO1
CSB-EP368414BPO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia Putative membrane protein insertion efficiency factor (Bcen2424_3164) CSB-YP368415BPO
CSB-EP368415BPO
CSB-BP368415BPO
CSB-MP368415BPO
CSB-EP368415BPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia Deoxyribose-phosphate aldolase (deoC) CSB-YP368416BPO
CSB-EP368416BPO
CSB-BP368416BPO
CSB-MP368416BPO
CSB-EP368416BPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia Putative ribose/galactose/methyl galactoside import ATP-binding protein 3 (Bcen2424_6709), partial CSB-YP368417BPO
CSB-EP368417BPO
CSB-BP368417BPO
CSB-MP368417BPO
CSB-EP368417BPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia Xylose isomerase (xylA) CSB-YP368418BPO
CSB-EP368418BPO
CSB-BP368418BPO
CSB-MP368418BPO
CSB-EP368418BPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas hydrophila subsp. hydrophila Chromosomal replication initiator protein DnaA (dnaA) CSB-YP368419AUH
CSB-EP368419AUH
CSB-BP368419AUH
CSB-MP368419AUH
CSB-EP368419AUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas hydrophila subsp. hydrophila Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase (gppA) CSB-YP368420AUH
CSB-EP368420AUH
CSB-BP368420AUH
CSB-MP368420AUH
CSB-EP368420AUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas hydrophila subsp. hydrophila Thiosulfate sulfurtransferase glpE (glpE) CSB-YP368421AUH
CSB-EP368421AUH
CSB-BP368421AUH
CSB-MP368421AUH
CSB-EP368421AUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas hydrophila subsp. hydrophila Sulfurtransferase TusA homolog (tusA) CSB-YP368422AUH
CSB-EP368422AUH
CSB-BP368422AUH
CSB-MP368422AUH
CSB-EP368422AUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas hydrophila subsp. hydrophila UPF0758 protein AHA_0160 (AHA_0160) CSB-YP368423AUH
CSB-EP368423AUH
CSB-BP368423AUH
CSB-MP368423AUH
CSB-EP368423AUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas hydrophila subsp. hydrophila Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP368424AUH
CSB-EP368424AUH
CSB-BP368424AUH
CSB-MP368424AUH
CSB-EP368424AUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas hydrophila subsp. hydrophila Glycerol-3-phosphate acyltransferase (plsB), partial CSB-YP368425AUH
CSB-EP368425AUH
CSB-BP368425AUH
CSB-MP368425AUH
CSB-EP368425AUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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