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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Burkholderia cenocepacia Sec-independent protein translocase protein TatA (tatA), partial CSB-YP368346BPO
CSB-EP368346BPO
CSB-BP368346BPO
CSB-MP368346BPO
CSB-EP368346BPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia 2-nonaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase (coq7) CSB-YP368347BPO
CSB-EP368347BPO
CSB-BP368347BPO
CSB-MP368347BPO
CSB-EP368347BPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD), partial CSB-YP368348BPO
CSB-EP368348BPO
CSB-BP368348BPO
CSB-MP368348BPO
CSB-EP368348BPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia Protein translocase subunit SecA (secA), partial CSB-YP368349BPO
CSB-EP368349BPO
CSB-BP368349BPO
CSB-MP368349BPO
CSB-EP368349BPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia GTPase obg (obg) CSB-YP368350BPO
CSB-EP368350BPO
CSB-BP368350BPO
CSB-MP368350BPO
CSB-EP368350BPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia Monofunctional biosynthetic peptidoglycan transglycosylase (mtgA), partial CSB-YP368351BPO1
CSB-EP368351BPO1
CSB-BP368351BPO1
CSB-MP368351BPO1
CSB-EP368351BPO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia Leucine--tRNA ligase (leuS), partial CSB-YP368352BPO
CSB-EP368352BPO
CSB-BP368352BPO
CSB-MP368352BPO
CSB-EP368352BPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia 30S ribosomal protein S9 (rpsI) CSB-YP368353BPO
CSB-EP368353BPO
CSB-BP368353BPO
CSB-MP368353BPO
CSB-EP368353BPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia D-tyrosyl-tRNA (Tyr) deacylase CSB-YP368354BPO
CSB-EP368354BPO
CSB-BP368354BPO
CSB-MP368354BPO
CSB-EP368354BPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP368355BPO
CSB-EP368355BPO
CSB-BP368355BPO
CSB-MP368355BPO
CSB-EP368355BPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia 4-hydroxybenzoate octaprenyltransferase (ubiA), partial CSB-YP368356BPO1
CSB-EP368356BPO1
CSB-BP368356BPO1
CSB-MP368356BPO1
CSB-EP368356BPO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia Protein grpE (grpE) CSB-YP368357BPO
CSB-EP368357BPO
CSB-BP368357BPO
CSB-MP368357BPO
CSB-EP368357BPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia 3-methyl-2-oxobutanoate hydroxymethyltransferase 1 (panB1) CSB-YP368358BPO
CSB-EP368358BPO
CSB-BP368358BPO
CSB-MP368358BPO
CSB-EP368358BPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia Transcriptional repressor NrdR (nrdR) CSB-YP368359BPO
CSB-EP368359BPO
CSB-BP368359BPO
CSB-MP368359BPO
CSB-EP368359BPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia UPF0301 protein Bcen2424_0864 (Bcen2424_0864) CSB-YP368360BPO
CSB-EP368360BPO
CSB-BP368360BPO
CSB-MP368360BPO
CSB-EP368360BPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia Urease accessory protein UreF (ureF) CSB-YP368361BPO
CSB-EP368361BPO
CSB-BP368361BPO
CSB-MP368361BPO
CSB-EP368361BPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia Urease subunit beta (ureB) CSB-YP368362BPO
CSB-EP368362BPO
CSB-BP368362BPO
CSB-MP368362BPO
CSB-EP368362BPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia N utilization substance protein B homolog (nusB) CSB-YP368363BPO
CSB-EP368363BPO
CSB-BP368363BPO
CSB-MP368363BPO
CSB-EP368363BPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia Cell division topological specificity factor (minE) CSB-YP368364BPO
CSB-EP368364BPO
CSB-BP368364BPO
CSB-MP368364BPO
CSB-EP368364BPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia Fructose-1,6-bisphosphatase class 1 (fbp) CSB-YP368365BPO
CSB-EP368365BPO
CSB-BP368365BPO
CSB-MP368365BPO
CSB-EP368365BPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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