Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Aeromonas hydrophila subsp. hydrophila DNA mismatch repair protein MutS (mutS), partial CSB-YP369066AUH
CSB-EP369066AUH
CSB-BP369066AUH
CSB-MP369066AUH
CSB-EP369066AUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas hydrophila subsp. hydrophila Bifunctional protein HldE (hldE) CSB-YP369067AUH
CSB-EP369067AUH
CSB-BP369067AUH
CSB-MP369067AUH
CSB-EP369067AUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas hydrophila subsp. hydrophila Probable Fe (2+)-trafficking protein CSB-YP369068AUH
CSB-EP369068AUH
CSB-BP369068AUH
CSB-MP369068AUH
CSB-EP369068AUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas hydrophila subsp. hydrophila Ribosomal RNA small subunit methyltransferase C (rsmC) CSB-YP369069AUH
CSB-EP369069AUH
CSB-BP369069AUH
CSB-MP369069AUH
CSB-EP369069AUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas hydrophila subsp. hydrophila UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (lpxC) CSB-YP369070AUH
CSB-EP369070AUH
CSB-BP369070AUH
CSB-MP369070AUH
CSB-EP369070AUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas hydrophila subsp. hydrophila UPF0102 protein AHA_3896 (AHA_3896) CSB-YP369071AUH
CSB-EP369071AUH
CSB-BP369071AUH
CSB-MP369071AUH
CSB-EP369071AUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas hydrophila subsp. hydrophila DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP369072AUH
CSB-EP369072AUH
CSB-BP369072AUH
CSB-MP369072AUH
CSB-EP369072AUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas hydrophila subsp. hydrophila 50S ribosomal protein L10 (rplJ) CSB-YP369073AUH
CSB-EP369073AUH
CSB-BP369073AUH
CSB-MP369073AUH
CSB-EP369073AUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas hydrophila subsp. hydrophila Aspartate carbamoyltransferase (pyrB) CSB-YP369074AUH
CSB-EP369074AUH
CSB-BP369074AUH
CSB-MP369074AUH
CSB-EP369074AUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas hydrophila subsp. hydrophila Undecaprenyl-diphosphatase (uppP), partial CSB-YP369075AUH
CSB-EP369075AUH
CSB-BP369075AUH
CSB-MP369075AUH
CSB-EP369075AUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas hydrophila subsp. hydrophila tRNA (uracil (54)-C (5))-methyltransferase CSB-YP369076AUH
CSB-EP369076AUH
CSB-BP369076AUH
CSB-MP369076AUH
CSB-EP369076AUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas hydrophila subsp. hydrophila tRNA modification GTPase MnmE (mnmE) CSB-YP369077AUH
CSB-EP369077AUH
CSB-BP369077AUH
CSB-MP369077AUH
CSB-EP369077AUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas hydrophila subsp. hydrophila 50S ribosomal protein L34 (rpmH) CSB-YP369078AUH
CSB-EP369078AUH
CSB-BP369078AUH
CSB-MP369078AUH
CSB-EP369078AUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP369079STS
CSB-EP369079STS
CSB-BP369079STS
CSB-MP369079STS
CSB-EP369079STS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Glycine--tRNA ligase beta subunit (glyS), partial CSB-YP369080STS
CSB-EP369080STS
CSB-BP369080STS
CSB-MP369080STS
CSB-EP369080STS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Fatty acid oxidation complex subunit alpha (fadB), partial CSB-YP369081STS
CSB-EP369081STS
CSB-BP369081STS
CSB-MP369081STS
CSB-EP369081STS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Coproporphyrinogen-III oxidase, aerobic (hemF) CSB-YP369082STS
CSB-EP369082STS
CSB-BP369082STS
CSB-MP369082STS
CSB-EP369082STS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Protein-export protein secB (secB) CSB-YP369083STS
CSB-EP369083STS
CSB-BP369083STS
CSB-MP369083STS
CSB-EP369083STS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Imidazolonepropionase (hutI) CSB-YP369084STS
CSB-EP369084STS
CSB-BP369084STS
CSB-MP369084STS
CSB-EP369084STS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. 3,4-dihydroxy-2-butanone 4-phosphate synthase (ribB) CSB-YP369085STS
CSB-EP369085STS
CSB-BP369085STS
CSB-MP369085STS
CSB-EP369085STS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>