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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Shewanella sp. Deoxycytidine triphosphate deaminase (dcd) CSB-YP369126STS
CSB-EP369126STS
CSB-BP369126STS
CSB-MP369126STS
CSB-EP369126STS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Ribosomal RNA small subunit methyltransferase F (rsmF) CSB-YP369127STS
CSB-EP369127STS
CSB-BP369127STS
CSB-MP369127STS
CSB-EP369127STS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP369128STS
CSB-EP369128STS
CSB-BP369128STS
CSB-MP369128STS
CSB-EP369128STS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Integration host factor subunit alpha (ihfA) CSB-YP369129STS
CSB-EP369129STS
CSB-BP369129STS
CSB-MP369129STS
CSB-EP369129STS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB) CSB-YP369130STS
CSB-EP369130STS
CSB-BP369130STS
CSB-MP369130STS
CSB-EP369130STS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Thiazole synthase (thiG) CSB-YP369131STS
CSB-EP369131STS
CSB-BP369131STS
CSB-MP369131STS
CSB-EP369131STS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP369132STS
CSB-EP369132STS
CSB-BP369132STS
CSB-MP369132STS
CSB-EP369132STS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Threonine--tRNA ligase (thrS), partial CSB-YP369133STS
CSB-EP369133STS
CSB-BP369133STS
CSB-MP369133STS
CSB-EP369133STS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Serine--tRNA ligase (serS) CSB-YP369134STS
CSB-EP369134STS
CSB-BP369134STS
CSB-MP369134STS
CSB-EP369134STS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Elongation factor P (efp) CSB-YP369135STS
CSB-EP369135STS
CSB-BP369135STS
CSB-MP369135STS
CSB-EP369135STS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Electron transport complex protein RnfD (rnfD), partial CSB-YP369136STS
CSB-EP369136STS
CSB-BP369136STS
CSB-MP369136STS
CSB-EP369136STS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. UPF0176 protein Shewana3_2261 (Shewana3_2261) CSB-YP369137STS
CSB-EP369137STS
CSB-BP369137STS
CSB-MP369137STS
CSB-EP369137STS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Chaperone protein hscA homolog (hscA), partial CSB-YP369138STS
CSB-EP369138STS
CSB-BP369138STS
CSB-MP369138STS
CSB-EP369138STS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Beta-hexosaminidase (nagZ) CSB-YP369139STS
CSB-EP369139STS
CSB-BP369139STS
CSB-MP369139STS
CSB-EP369139STS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Adenylate kinase (adk) CSB-YP369140STS
CSB-EP369140STS
CSB-BP369140STS
CSB-MP369140STS
CSB-EP369140STS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Biotin synthase (bioB) CSB-YP369141STS
CSB-EP369141STS
CSB-BP369141STS
CSB-MP369141STS
CSB-EP369141STS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Ribonuclease T (rnt) CSB-YP369142STS
CSB-EP369142STS
CSB-BP369142STS
CSB-MP369142STS
CSB-EP369142STS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. GTP cyclohydrolase-2 (ribA) CSB-YP369143STS
CSB-EP369143STS
CSB-BP369143STS
CSB-MP369143STS
CSB-EP369143STS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Bifunctional protein FolD (folD) CSB-YP369144STS
CSB-EP369144STS
CSB-BP369144STS
CSB-MP369144STS
CSB-EP369144STS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Glycosyl hydrolase family 109 protein 2 (Shewana3_2701) CSB-YP369145STS
CSB-EP369145STS
CSB-BP369145STS
CSB-MP369145STS
CSB-EP369145STS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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