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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Francisella tularensis subsp. novicida Ribonuclease PH (rph) CSB-YP369626FDW
CSB-EP369626FDW
CSB-BP369626FDW
CSB-MP369626FDW
CSB-EP369626FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. novicida Bifunctional protein FolD (folD) CSB-YP369627FDW
CSB-EP369627FDW
CSB-BP369627FDW
CSB-MP369627FDW
CSB-EP369627FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. novicida GTPase Der (der) CSB-YP369628FDW
CSB-EP369628FDW
CSB-BP369628FDW
CSB-MP369628FDW
CSB-EP369628FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. novicida Methionine--tRNA ligase (metG), partial CSB-YP369629FDW
CSB-EP369629FDW
CSB-BP369629FDW
CSB-MP369629FDW
CSB-EP369629FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. novicida Glucose-1-phosphate adenylyltransferase (glgC) CSB-YP369630FDW
CSB-EP369630FDW
CSB-BP369630FDW
CSB-MP369630FDW
CSB-EP369630FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. novicida Glycine--tRNA ligase alpha subunit (glyQ) CSB-YP369631FDW
CSB-EP369631FDW
CSB-BP369631FDW
CSB-MP369631FDW
CSB-EP369631FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. novicida Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP369632FDW
CSB-EP369632FDW
CSB-BP369632FDW
CSB-MP369632FDW
CSB-EP369632FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. novicida Arginine--tRNA ligase (argS), partial CSB-YP369633FDW
CSB-EP369633FDW
CSB-BP369633FDW
CSB-MP369633FDW
CSB-EP369633FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. novicida 3-dehydroquinate dehydratase (aroQ) CSB-YP369634FDW
CSB-EP369634FDW
CSB-BP369634FDW
CSB-MP369634FDW
CSB-EP369634FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. novicida 30S ribosomal protein S15 (rpsO) CSB-YP369635FDW
CSB-EP369635FDW
CSB-BP369635FDW
CSB-MP369635FDW
CSB-EP369635FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. novicida Iron-sulfur cluster insertion protein ErpA (erpA) CSB-YP369636FDW
CSB-EP369636FDW
CSB-BP369636FDW
CSB-MP369636FDW
CSB-EP369636FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. novicida 2-dehydro-3-deoxyphosphooctonate aldolase (kdsA) CSB-YP369637FDW
CSB-EP369637FDW
CSB-BP369637FDW
CSB-MP369637FDW
CSB-EP369637FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. novicida Enolase (eno) CSB-YP369638FDW
CSB-EP369638FDW
CSB-BP369638FDW
CSB-MP369638FDW
CSB-EP369638FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. novicida Uracil phosphoribosyltransferase (upp) CSB-YP369639FDW
CSB-EP369639FDW
CSB-BP369639FDW
CSB-MP369639FDW
CSB-EP369639FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. novicida Catalase-peroxidase (katG), partial CSB-YP369640FDW
CSB-EP369640FDW
CSB-BP369640FDW
CSB-MP369640FDW
CSB-EP369640FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. novicida UvrABC system protein C (uvrC), partial CSB-YP369641FDW
CSB-EP369641FDW
CSB-BP369641FDW
CSB-MP369641FDW
CSB-EP369641FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. novicida 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB) CSB-YP369642FDW
CSB-EP369642FDW
CSB-BP369642FDW
CSB-MP369642FDW
CSB-EP369642FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. novicida DNA replication and repair protein recF (recF) CSB-YP369643FDW
CSB-EP369643FDW
CSB-BP369643FDW
CSB-MP369643FDW
CSB-EP369643FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. novicida Alanine--tRNA ligase (alaS), partial CSB-YP369644FDW
CSB-EP369644FDW
CSB-BP369644FDW
CSB-MP369644FDW
CSB-EP369644FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. novicida Biotin synthase (bioB) CSB-YP369645FDW
CSB-EP369645FDW
CSB-BP369645FDW
CSB-MP369645FDW
CSB-EP369645FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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