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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Clostridium novyi DNA repair protein recO (recO) CSB-YP369586CWY
CSB-EP369586CWY
CSB-BP369586CWY
CSB-MP369586CWY
CSB-EP369586CWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium novyi Ribosomal protein L11 methyltransferase (prmA) CSB-YP369587CWY
CSB-EP369587CWY
CSB-BP369587CWY
CSB-MP369587CWY
CSB-EP369587CWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium novyi Chaperone protein DnaK (dnaK), partial CSB-YP369588CWY
CSB-EP369588CWY
CSB-BP369588CWY
CSB-MP369588CWY
CSB-EP369588CWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium novyi Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP369589CWY
CSB-EP369589CWY
CSB-BP369589CWY
CSB-MP369589CWY
CSB-EP369589CWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium novyi Quinolinate synthase A (nadA) CSB-YP369590CWY
CSB-EP369590CWY
CSB-BP369590CWY
CSB-MP369590CWY
CSB-EP369590CWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium novyi Undecaprenyl-diphosphatase (uppP), partial CSB-YP369591CWY1
CSB-EP369591CWY1
CSB-BP369591CWY1
CSB-MP369591CWY1
CSB-EP369591CWY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium novyi Hydroxyethylthiazole kinase (thiM) CSB-YP369592CWY
CSB-EP369592CWY
CSB-BP369592CWY
CSB-MP369592CWY
CSB-EP369592CWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium novyi Proline--tRNA ligase (proS) CSB-YP369593CWY
CSB-EP369593CWY
CSB-BP369593CWY
CSB-MP369593CWY
CSB-EP369593CWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium novyi tRNA (guanine-N (7)-)-methyltransferase CSB-YP369594CWY
CSB-EP369594CWY
CSB-BP369594CWY
CSB-MP369594CWY
CSB-EP369594CWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium novyi ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP369595CWY
CSB-EP369595CWY
CSB-BP369595CWY
CSB-MP369595CWY
CSB-EP369595CWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium novyi Aspartate carbamoyltransferase (pyrB) CSB-YP369596CWY
CSB-EP369596CWY
CSB-BP369596CWY
CSB-MP369596CWY
CSB-EP369596CWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium novyi Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP369597CWY
CSB-EP369597CWY
CSB-BP369597CWY
CSB-MP369597CWY
CSB-EP369597CWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium novyi 60 kDa chaperonin (groL), partial CSB-YP369598CWY
CSB-EP369598CWY
CSB-BP369598CWY
CSB-MP369598CWY
CSB-EP369598CWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium novyi Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP369599CWY
CSB-EP369599CWY
CSB-BP369599CWY
CSB-MP369599CWY
CSB-EP369599CWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium novyi 50S ribosomal protein L31 (rpmE) CSB-YP369600CWY
CSB-EP369600CWY
CSB-BP369600CWY
CSB-MP369600CWY
CSB-EP369600CWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium novyi 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP369601CWY
CSB-EP369601CWY
CSB-BP369601CWY
CSB-MP369601CWY
CSB-EP369601CWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium novyi 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP369602CWY
CSB-EP369602CWY
CSB-BP369602CWY
CSB-MP369602CWY
CSB-EP369602CWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium novyi Homoserine kinase (thrB) CSB-YP369603CWY
CSB-EP369603CWY
CSB-BP369603CWY
CSB-MP369603CWY
CSB-EP369603CWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium novyi Riboflavin biosynthesis protein ribBA (ribBA) CSB-YP369604CWY
CSB-EP369604CWY
CSB-BP369604CWY
CSB-MP369604CWY
CSB-EP369604CWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium novyi 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP369605CWY
CSB-EP369605CWY
CSB-BP369605CWY
CSB-MP369605CWY
CSB-EP369605CWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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