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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Shewanella sp. DNA mismatch repair protein MutS (mutS), partial CSB-YP370367STS
CSB-EP370367STS
CSB-BP370367STS
CSB-MP370367STS
CSB-EP370367STS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. UPF0312 protein Shewana3_1179 (Shewana3_1179) CSB-YP370368STS
CSB-EP370368STS
CSB-BP370368STS
CSB-MP370368STS
CSB-EP370368STS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP370369STS
CSB-EP370369STS
CSB-BP370369STS
CSB-MP370369STS
CSB-EP370369STS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. GTPase Der (der) CSB-YP370370STS
CSB-EP370370STS
CSB-BP370370STS
CSB-MP370370STS
CSB-EP370370STS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Proline--tRNA ligase (proS), partial CSB-YP370371STS
CSB-EP370371STS
CSB-BP370371STS
CSB-MP370371STS
CSB-EP370371STS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP370372STS
CSB-EP370372STS
CSB-BP370372STS
CSB-MP370372STS
CSB-EP370372STS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Fatty acid oxidation complex subunit alpha (fadJ), partial CSB-YP370373STS
CSB-EP370373STS
CSB-BP370373STS
CSB-MP370373STS
CSB-EP370373STS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Erythronate-4-phosphate dehydrogenase (pdxB) CSB-YP370374STS
CSB-EP370374STS
CSB-BP370374STS
CSB-MP370374STS
CSB-EP370374STS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. DNA ligase (ligA), partial CSB-YP370375STS
CSB-EP370375STS
CSB-BP370375STS
CSB-MP370375STS
CSB-EP370375STS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. UPF0178 protein Shewana3_1627 (Shewana3_1627) CSB-YP370376STS
CSB-EP370376STS
CSB-BP370376STS
CSB-MP370376STS
CSB-EP370376STS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Dihydrodipicolinate synthase (dapA) CSB-YP370377STS
CSB-EP370377STS
CSB-BP370377STS
CSB-MP370377STS
CSB-EP370377STS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Putative phosphotransferase Shewana3_1735 (Shewana3_1735) CSB-YP370378STS
CSB-EP370378STS
CSB-BP370378STS
CSB-MP370378STS
CSB-EP370378STS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. ATP-dependent Clp protease adapter protein ClpS (clpS) CSB-YP370379STS
CSB-EP370379STS
CSB-BP370379STS
CSB-MP370379STS
CSB-EP370379STS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Putative arginyl-tRNA--protein transferase (ate) CSB-YP370380STS
CSB-EP370380STS
CSB-BP370380STS
CSB-MP370380STS
CSB-EP370380STS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP370381STS
CSB-EP370381STS
CSB-BP370381STS
CSB-MP370381STS
CSB-EP370381STS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Succinyl-diaminopimelate desuccinylase (dapE) CSB-YP370382STS
CSB-EP370382STS
CSB-BP370382STS
CSB-MP370382STS
CSB-EP370382STS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Aspartate--tRNA ligase (aspS), partial CSB-YP370383STS
CSB-EP370383STS
CSB-BP370383STS
CSB-MP370383STS
CSB-EP370383STS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP370384STS
CSB-EP370384STS
CSB-BP370384STS
CSB-MP370384STS
CSB-EP370384STS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP370385STS
CSB-EP370385STS
CSB-BP370385STS
CSB-MP370385STS
CSB-EP370385STS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Integration host factor subunit beta (ihfB) CSB-YP370386STS
CSB-EP370386STS
CSB-BP370386STS
CSB-MP370386STS
CSB-EP370386STS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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