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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Glutamate 5-kinase (proB) CSB-YP372168EJE
CSB-EP372168EJE
CSB-BP372168EJE
CSB-MP372168EJE
CSB-EP372168EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein matC (matC) CSB-YP372169EJE
CSB-EP372169EJE
CSB-BP372169EJE
CSB-MP372169EJE
CSB-EP372169EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable HTH-type transcriptional regulator matA homolog (matA) CSB-YP372170EJE
CSB-EP372170EJE
CSB-BP372170EJE
CSB-MP372170EJE
CSB-EP372170EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant S-formylglutathione hydrolase frmB (frmB) CSB-YP372171EJE
CSB-EP372171EJE
CSB-BP372171EJE
CSB-MP372171EJE
CSB-EP372171EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Transcriptional repressor frmR (frmR) CSB-YP372172EJE
CSB-EP372172EJE
CSB-BP372172EJE
CSB-MP372172EJE
CSB-EP372172EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0178 protein yaiI (yaiI) CSB-YP372173EJE
CSB-EP372173EJE
CSB-BP372173EJE
CSB-MP372173EJE
CSB-EP372173EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0345 protein YaiE (yaiE) CSB-YP372174EJE
CSB-EP372174EJE
CSB-BP372174EJE
CSB-MP372174EJE
CSB-EP372174EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 1-deoxy-D-xylulose-5-phosphate synthase (dxs) CSB-YP372175EJE
CSB-EP372175EJE
CSB-BP372175EJE
CSB-MP372175EJE
CSB-EP372175EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP372176EJE
CSB-EP372176EJE
CSB-BP372176EJE
CSB-MP372176EJE
CSB-EP372176EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 7-cyano-7-deazaguanine synthase (queC) CSB-YP372177EJE
CSB-EP372177EJE
CSB-BP372177EJE
CSB-MP372177EJE
CSB-EP372177EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Recombination protein RecR (recR) CSB-YP372178EJE
CSB-EP372178EJE
CSB-BP372178EJE
CSB-MP372178EJE
CSB-EP372178EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant tRNA 2-selenouridine synthase (selU) CSB-YP372179EJE
CSB-EP372179EJE
CSB-BP372179EJE
CSB-MP372179EJE
CSB-EP372179EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant HTH-type transcriptional repressor AllR (allR) CSB-YP372180EJE
CSB-EP372180EJE
CSB-BP372180EJE
CSB-MP372180EJE
CSB-EP372180EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UDP-2,3-diacylglucosamine hydrolase (lpxH) CSB-YP372181EJE
CSB-EP372181EJE
CSB-BP372181EJE
CSB-MP372181EJE
CSB-EP372181EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cysteine--tRNA ligase (cysS) CSB-YP372182EJE
CSB-EP372182EJE
CSB-BP372182EJE
CSB-MP372182EJE
CSB-EP372182EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 2- (5''-triphosphoribosyl)-3'-dephosphocoenzyme-A synthase CSB-YP372183EJE
CSB-EP372183EJE
CSB-BP372183EJE
CSB-MP372183EJE
CSB-EP372183EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP372184EJE
CSB-EP372184EJE
CSB-BP372184EJE
CSB-MP372184EJE
CSB-EP372184EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant LPS-assembly lipoprotein lptE (lptE) CSB-YP372185EJE
CSB-EP372185EJE
CSB-BP372185EJE
CSB-MP372185EJE
CSB-EP372185EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrimidine-specific ribonucleoside hydrolase RihA (rihA) CSB-YP372186EJE
CSB-EP372186EJE
CSB-BP372186EJE
CSB-MP372186EJE
CSB-EP372186EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP372187EJE
CSB-EP372187EJE
CSB-BP372187EJE
CSB-MP372187EJE
CSB-EP372187EJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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