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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Mycobacterium ulcerans Pup--protein ligase (pafA) CSB-YP370707MOP
CSB-EP370707MOP
CSB-BP370707MOP
CSB-MP370707MOP
CSB-EP370707MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans Prokaryotic ubiquitin-like protein Pup (pup) CSB-YP370708MOP
CSB-EP370708MOP
CSB-BP370708MOP
CSB-MP370708MOP
CSB-EP370708MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans Undecaprenyl-diphosphatase (uppP), partial CSB-YP370709MOP1
CSB-EP370709MOP1
CSB-BP370709MOP1
CSB-MP370709MOP1
CSB-EP370709MOP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans Dihydroorotate dehydrogenase (quinone) CSB-YP370710MOP
CSB-EP370710MOP
CSB-BP370710MOP
CSB-MP370710MOP
CSB-EP370710MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans Peptide chain release factor 2 (prfB) CSB-YP370711MOP
CSB-EP370711MOP
CSB-BP370711MOP
CSB-MP370711MOP
CSB-EP370711MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans NADH-quinone oxidoreductase subunit D (nuoD) CSB-YP370712MOP
CSB-EP370712MOP
CSB-BP370712MOP
CSB-MP370712MOP
CSB-EP370712MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans NADH-quinone oxidoreductase subunit K (nuoK), partial CSB-YP370713MOP1
CSB-EP370713MOP1
CSB-BP370713MOP1
CSB-MP370713MOP1
CSB-EP370713MOP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans Lipoprotein lpqB (lpqB), partial CSB-YP370714MOP
CSB-EP370714MOP
CSB-BP370714MOP
CSB-MP370714MOP
CSB-EP370714MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans Maf-like protein MUL_2634 (MUL_2634) CSB-YP370715MOP
CSB-EP370715MOP
CSB-BP370715MOP
CSB-MP370715MOP
CSB-EP370715MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans Thiamine-phosphate synthase (thiE) CSB-YP370716MOP
CSB-EP370716MOP
CSB-BP370716MOP
CSB-MP370716MOP
CSB-EP370716MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans Putative S-adenosyl-L-methionine-dependent methyltransferase MUL_2961 (MUL_2961) CSB-YP370717MOP
CSB-EP370717MOP
CSB-BP370717MOP
CSB-MP370717MOP
CSB-EP370717MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans Urease accessory protein UreG (ureG) CSB-YP370718MOP
CSB-EP370718MOP
CSB-BP370718MOP
CSB-MP370718MOP
CSB-EP370718MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans Urease subunit gamma (ureA) CSB-YP370719MOP
CSB-EP370719MOP
CSB-BP370719MOP
CSB-MP370719MOP
CSB-EP370719MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans Putative succinate-semialdehyde dehydrogenase [NADP (+)] 2, partial CSB-YP370720MOP
CSB-EP370720MOP
CSB-BP370720MOP
CSB-MP370720MOP
CSB-EP370720MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans GTPase Der (der) CSB-YP370721MOP
CSB-EP370721MOP
CSB-BP370721MOP
CSB-MP370721MOP
CSB-EP370721MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans Threonine--tRNA ligase (thrS), partial CSB-YP370722MOP
CSB-EP370722MOP
CSB-BP370722MOP
CSB-MP370722MOP
CSB-EP370722MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut) CSB-YP370723MOP
CSB-EP370723MOP
CSB-BP370723MOP
CSB-MP370723MOP
CSB-EP370723MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans Diaminopimelate epimerase (dapF) CSB-YP370724MOP
CSB-EP370724MOP
CSB-BP370724MOP
CSB-MP370724MOP
CSB-EP370724MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans Regulatory protein recX (recX) CSB-YP370725MOP
CSB-EP370725MOP
CSB-BP370725MOP
CSB-MP370725MOP
CSB-EP370725MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans tRNA dimethylallyltransferase 2 (miaA2) CSB-YP370726MOP
CSB-EP370726MOP
CSB-BP370726MOP
CSB-MP370726MOP
CSB-EP370726MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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