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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Mycobacterium ulcerans Tryptophan synthase alpha chain (trpA) CSB-YP370687MOP
CSB-EP370687MOP
CSB-BP370687MOP
CSB-MP370687MOP
CSB-EP370687MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans Lysylphosphatidylglycerol biosynthesis bifunctional protein LysX (lysX), partial CSB-YP370688MOP
CSB-EP370688MOP
CSB-BP370688MOP
CSB-MP370688MOP
CSB-EP370688MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans Acetylglutamate kinase (argB) CSB-YP370689MOP
CSB-EP370689MOP
CSB-BP370689MOP
CSB-MP370689MOP
CSB-EP370689MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP370690MOP
CSB-EP370690MOP
CSB-BP370690MOP
CSB-MP370690MOP
CSB-EP370690MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans Histidine--tRNA ligase (hisS) CSB-YP370691MOP
CSB-EP370691MOP
CSB-BP370691MOP
CSB-MP370691MOP
CSB-EP370691MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans Shikimate kinase (aroK) CSB-YP370692MOP
CSB-EP370692MOP
CSB-BP370692MOP
CSB-MP370692MOP
CSB-EP370692MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans Aspartate carbamoyltransferase (pyrB) CSB-YP370693MOP
CSB-EP370693MOP
CSB-BP370693MOP
CSB-MP370693MOP
CSB-EP370693MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans Putative sporulation transcription regulator WhiA (whiA) CSB-YP370694MOP
CSB-EP370694MOP
CSB-BP370694MOP
CSB-MP370694MOP
CSB-EP370694MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP370695MOP
CSB-EP370695MOP
CSB-BP370695MOP
CSB-MP370695MOP
CSB-EP370695MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C CSB-YP370696MOP
CSB-EP370696MOP
CSB-BP370696MOP
CSB-MP370696MOP
CSB-EP370696MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans Ketol-acid reductoisomerase (ilvC) CSB-YP370697MOP
CSB-EP370697MOP
CSB-BP370697MOP
CSB-MP370697MOP
CSB-EP370697MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans 3-isopropylmalate dehydrogenase (leuB) CSB-YP370698MOP
CSB-EP370698MOP
CSB-BP370698MOP
CSB-MP370698MOP
CSB-EP370698MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP370699MOP
CSB-EP370699MOP
CSB-BP370699MOP
CSB-MP370699MOP
CSB-EP370699MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans Probable phthiotriol/phenolphthiotriol dimycocerosates methyltransferase 2 (MUL_2009) CSB-YP370700MOP
CSB-EP370700MOP
CSB-BP370700MOP
CSB-MP370700MOP
CSB-EP370700MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP370701MOP
CSB-EP370701MOP
CSB-BP370701MOP
CSB-MP370701MOP
CSB-EP370701MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans UPF0102 protein MUL_2060 (MUL_2060) CSB-YP370702MOP
CSB-EP370702MOP
CSB-BP370702MOP
CSB-MP370702MOP
CSB-EP370702MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP370703MOP
CSB-EP370703MOP
CSB-BP370703MOP
CSB-MP370703MOP
CSB-EP370703MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans Proline--tRNA ligase (proS), partial CSB-YP370704MOP
CSB-EP370704MOP
CSB-BP370704MOP
CSB-MP370704MOP
CSB-EP370704MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans tRNA pseudouridine synthase B (truB) CSB-YP370705MOP
CSB-EP370705MOP
CSB-BP370705MOP
CSB-MP370705MOP
CSB-EP370705MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP370706MOP
CSB-EP370706MOP
CSB-BP370706MOP
CSB-MP370706MOP
CSB-EP370706MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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