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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Paracoccus denitrificans D-amino acid dehydrogenase small subunit (dadA) CSB-YP372588PYC
CSB-EP372588PYC
CSB-BP372588PYC
CSB-MP372588PYC
CSB-EP372588PYC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccus denitrificans tRNA 2-thiocytidine biosynthesis protein TtcA (ttcA) CSB-YP372589PYC
CSB-EP372589PYC
CSB-BP372589PYC
CSB-MP372589PYC
CSB-EP372589PYC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccus denitrificans Trigger factor (tig) CSB-YP372590PYC
CSB-EP372590PYC
CSB-BP372590PYC
CSB-MP372590PYC
CSB-EP372590PYC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccus denitrificans Deoxyguanosinetriphosphate triphosphohydrolase-like protein (Pden_0927) CSB-YP372591PYC
CSB-EP372591PYC
CSB-BP372591PYC
CSB-MP372591PYC
CSB-EP372591PYC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccus denitrificans Urease accessory protein UreE (ureE) CSB-YP372592PYC
CSB-EP372592PYC
CSB-BP372592PYC
CSB-MP372592PYC
CSB-EP372592PYC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccus denitrificans UDP-3-O-acylglucosamine N-acyltransferase (lpxD) CSB-YP372593PYC
CSB-EP372593PYC
CSB-BP372593PYC
CSB-MP372593PYC
CSB-EP372593PYC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccus denitrificans 3-dehydroquinate synthase (aroB) CSB-YP372594PYC
CSB-EP372594PYC
CSB-BP372594PYC
CSB-MP372594PYC
CSB-EP372594PYC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccus denitrificans DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP372595PYC
CSB-EP372595PYC
CSB-BP372595PYC
CSB-MP372595PYC
CSB-EP372595PYC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccus denitrificans Transcriptional repressor NrdR (nrdR) CSB-YP372596PYC
CSB-EP372596PYC
CSB-BP372596PYC
CSB-MP372596PYC
CSB-EP372596PYC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccus denitrificans Pantothenate synthetase (panC) CSB-YP372597PYC
CSB-EP372597PYC
CSB-BP372597PYC
CSB-MP372597PYC
CSB-EP372597PYC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccus denitrificans Pseudouridine-5'-phosphate glycosidase (psuG) CSB-YP372598PYC
CSB-EP372598PYC
CSB-BP372598PYC
CSB-MP372598PYC
CSB-EP372598PYC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccus denitrificans 50S ribosomal protein L32 (rpmF) CSB-YP372599PYC
CSB-EP372599PYC
CSB-BP372599PYC
CSB-MP372599PYC
CSB-EP372599PYC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccus denitrificans Integration host factor subunit alpha (ihfA) CSB-YP372600PYC
CSB-EP372600PYC
CSB-BP372600PYC
CSB-MP372600PYC
CSB-EP372600PYC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccus denitrificans 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP372601PYC
CSB-EP372601PYC
CSB-BP372601PYC
CSB-MP372601PYC
CSB-EP372601PYC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccus denitrificans Tyrosine--tRNA ligase (tyrS) CSB-YP372602PYC
CSB-EP372602PYC
CSB-BP372602PYC
CSB-MP372602PYC
CSB-EP372602PYC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccus denitrificans Octanoyltransferase (lipB) CSB-YP372603PYC
CSB-EP372603PYC
CSB-BP372603PYC
CSB-MP372603PYC
CSB-EP372603PYC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccus denitrificans Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP372604PYC
CSB-EP372604PYC
CSB-BP372604PYC
CSB-MP372604PYC
CSB-EP372604PYC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccus denitrificans Methionine--tRNA ligase (metG), partial CSB-YP372605PYC
CSB-EP372605PYC
CSB-BP372605PYC
CSB-MP372605PYC
CSB-EP372605PYC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccus denitrificans LexA repressor (lexA) CSB-YP372606PYC
CSB-EP372606PYC
CSB-BP372606PYC
CSB-MP372606PYC
CSB-EP372606PYC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccus denitrificans Uracil phosphoribosyltransferase (upp) CSB-YP372607PYC
CSB-EP372607PYC
CSB-BP372607PYC
CSB-MP372607PYC
CSB-EP372607PYC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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