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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Aspergillus clavatus Dol-P-Man:Man (5)GlcNAc (2)-PP-Dol alpha-1,3-mannosyltransferase, partial CSB-YP372988AVC
CSB-EP372988AVC
CSB-BP372988AVC
CSB-MP372988AVC
CSB-EP372988AVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus clavatus Mitochondrial division protein 1 (mdv1), partial CSB-YP372989AVC
CSB-EP372989AVC
CSB-BP372989AVC
CSB-MP372989AVC
CSB-EP372989AVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus clavatus Mitochondrial distribution and morphology protein 10 (mdmB) CSB-YP372990AVC
CSB-EP372990AVC
CSB-BP372990AVC
CSB-MP372990AVC
CSB-EP372990AVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus clavatus Probable alpha-glucuronidase A (aguA), partial CSB-YP372991AVC
CSB-EP372991AVC
CSB-BP372991AVC
CSB-MP372991AVC
CSB-EP372991AVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus clavatus Probable exo-1,4-beta-xylosidase bxlB (bxlB), partial CSB-YP372992AVC
CSB-EP372992AVC
CSB-BP372992AVC
CSB-MP372992AVC
CSB-EP372992AVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus clavatus Methionine aminopeptidase 2 homolog ACLA_062640 (ACLA_062640) CSB-YP372993AVC
CSB-EP372993AVC
CSB-BP372993AVC
CSB-MP372993AVC
CSB-EP372993AVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus clavatus Probable glucan endo-1,3-beta-glucosidase eglC (eglC) CSB-YP372994AVC
CSB-EP372994AVC
CSB-BP372994AVC
CSB-MP372994AVC
CSB-EP372994AVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus clavatus Actin cytoskeleton-regulatory complex protein pan1 (pan1), partial CSB-YP372995AVC
CSB-EP372995AVC
CSB-BP372995AVC
CSB-MP372995AVC
CSB-EP372995AVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus clavatus DNA damage-inducible protein 1 (ddi1) CSB-YP372996AVC
CSB-EP372996AVC
CSB-BP372996AVC
CSB-MP372996AVC
CSB-EP372996AVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus clavatus Probable beta-galactosidase C (lacC), partial CSB-YP372997AVC
CSB-EP372997AVC
CSB-BP372997AVC
CSB-MP372997AVC
CSB-EP372997AVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus clavatus Probable carboxypeptidase ACLA_088580 (ACLA_088580) CSB-YP372998AVC
CSB-EP372998AVC
CSB-BP372998AVC
CSB-MP372998AVC
CSB-EP372998AVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus clavatus Histone chaperone rtt106 (rtt106) CSB-YP372999AVC
CSB-EP372999AVC
CSB-BP372999AVC
CSB-MP372999AVC
CSB-EP372999AVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus clavatus Probable 1,4-beta-D-glucan cellobiohydrolase A (cbhA) CSB-YP373000AVC
CSB-EP373000AVC
CSB-BP373000AVC
CSB-MP373000AVC
CSB-EP373000AVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus clavatus Vacuolar fusion protein mon1 (mon1), partial CSB-YP373001AVC
CSB-EP373001AVC
CSB-BP373001AVC
CSB-MP373001AVC
CSB-EP373001AVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus clavatus Class E vacuolar protein-sorting machinery protein hse1 (hse1), partial CSB-YP373002AVC
CSB-EP373002AVC
CSB-BP373002AVC
CSB-MP373002AVC
CSB-EP373002AVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus clavatus Protein cgi121 (cgi121) CSB-YP373003AVC
CSB-EP373003AVC
CSB-BP373003AVC
CSB-MP373003AVC
CSB-EP373003AVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus clavatus Nuclear distribution protein nudF 2 (nudF-2) CSB-YP373004AVC
CSB-EP373004AVC
CSB-BP373004AVC
CSB-MP373004AVC
CSB-EP373004AVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus clavatus Polynucleotide 5'-hydroxyl-kinase grc3 (grc3), partial CSB-YP373005AVC
CSB-EP373005AVC
CSB-BP373005AVC
CSB-MP373005AVC
CSB-EP373005AVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus clavatus Probable pectin lyase A (pelA) CSB-YP373006AVC
CSB-EP373006AVC
CSB-BP373006AVC
CSB-MP373006AVC
CSB-EP373006AVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus clavatus Probable D-xylulose reductase A (xdhA) CSB-YP373007AVC
CSB-EP373007AVC
CSB-BP373007AVC
CSB-MP373007AVC
CSB-EP373007AVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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