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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Pelobacter propionicus Probable potassium transport system protein kup (kup), partial CSB-YP373408PYF
CSB-EP373408PYF
CSB-BP373408PYF
CSB-MP373408PYF
CSB-EP373408PYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelobacter propionicus UPF0272 protein Ppro_0626 (Ppro_0626) CSB-YP373409PYF
CSB-EP373409PYF
CSB-BP373409PYF
CSB-MP373409PYF
CSB-EP373409PYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelobacter propionicus NADH-quinone oxidoreductase subunit I (nuoI1) CSB-YP373410PYF
CSB-EP373410PYF
CSB-BP373410PYF
CSB-MP373410PYF
CSB-EP373410PYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelobacter propionicus 50S ribosomal protein L11 (rplK) CSB-YP373411PYF
CSB-EP373411PYF
CSB-BP373411PYF
CSB-MP373411PYF
CSB-EP373411PYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelobacter propionicus DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP373412PYF
CSB-EP373412PYF
CSB-BP373412PYF
CSB-MP373412PYF
CSB-EP373412PYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelobacter propionicus 30S ribosomal protein S10 (rpsJ) CSB-YP373413PYF
CSB-EP373413PYF
CSB-BP373413PYF
CSB-MP373413PYF
CSB-EP373413PYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelobacter propionicus 50S ribosomal protein L16 (rplP) CSB-YP373414PYF
CSB-EP373414PYF
CSB-BP373414PYF
CSB-MP373414PYF
CSB-EP373414PYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelobacter propionicus 30S ribosomal protein S14 type Z (rpsZ) CSB-YP373415PYF
CSB-EP373415PYF
CSB-BP373415PYF
CSB-MP373415PYF
CSB-EP373415PYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelobacter propionicus 50S ribosomal protein L18 (rplR) CSB-YP373416PYF
CSB-EP373416PYF
CSB-BP373416PYF
CSB-MP373416PYF
CSB-EP373416PYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelobacter propionicus Adenylate kinase (adk) CSB-YP373417PYF
CSB-EP373417PYF
CSB-BP373417PYF
CSB-MP373417PYF
CSB-EP373417PYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelobacter propionicus DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP373418PYF
CSB-EP373418PYF
CSB-BP373418PYF
CSB-MP373418PYF
CSB-EP373418PYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelobacter propionicus 50S ribosomal protein L25 (rplY) CSB-YP373419PYF
CSB-EP373419PYF
CSB-BP373419PYF
CSB-MP373419PYF
CSB-EP373419PYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelobacter propionicus 30S ribosomal protein S18 (rpsR) CSB-YP373420PYF
CSB-EP373420PYF
CSB-BP373420PYF
CSB-MP373420PYF
CSB-EP373420PYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelobacter propionicus Cobalamin biosynthesis protein CobD (cobD), partial CSB-YP373421PYF1
CSB-EP373421PYF1
CSB-BP373421PYF1
CSB-MP373421PYF1
CSB-EP373421PYF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelobacter propionicus UPF0042 nucleotide-binding protein Ppro_0977 (Ppro_0977) CSB-YP373422PYF
CSB-EP373422PYF
CSB-BP373422PYF
CSB-MP373422PYF
CSB-EP373422PYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelobacter propionicus Ribonuclease HII (rnhB) CSB-YP373423PYF
CSB-EP373423PYF
CSB-BP373423PYF
CSB-MP373423PYF
CSB-EP373423PYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelobacter propionicus Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP373424PYF
CSB-EP373424PYF
CSB-BP373424PYF
CSB-MP373424PYF
CSB-EP373424PYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelobacter propionicus ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP373425PYF
CSB-EP373425PYF
CSB-BP373425PYF
CSB-MP373425PYF
CSB-EP373425PYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelobacter propionicus Cobalt transport protein CbiM 2 (cbiM2), partial CSB-YP373426PYF1
CSB-EP373426PYF1
CSB-BP373426PYF1
CSB-MP373426PYF1
CSB-EP373426PYF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelobacter propionicus 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP373427PYF
CSB-EP373427PYF
CSB-BP373427PYF
CSB-MP373427PYF
CSB-EP373427PYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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