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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Chlorobium phaeobacteroides Thiamine-phosphate synthase (thiE) CSB-YP373608CWO
CSB-EP373608CWO
CSB-BP373608CWO
CSB-MP373608CWO
CSB-EP373608CWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides ATP-dependent protease subunit HslV (hslV) CSB-YP373609CWO
CSB-EP373609CWO
CSB-BP373609CWO
CSB-MP373609CWO
CSB-EP373609CWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides Aspartate--tRNA ligase (aspS), partial CSB-YP373610CWO
CSB-EP373610CWO
CSB-BP373610CWO
CSB-MP373610CWO
CSB-EP373610CWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides 50S ribosomal protein L25 (rplY) CSB-YP373611CWO
CSB-EP373611CWO
CSB-BP373611CWO
CSB-MP373611CWO
CSB-EP373611CWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides Serine--tRNA ligase (serS) CSB-YP373612CWO
CSB-EP373612CWO
CSB-BP373612CWO
CSB-MP373612CWO
CSB-EP373612CWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides Chorismate synthase (aroC) CSB-YP373613CWO
CSB-EP373613CWO
CSB-BP373613CWO
CSB-MP373613CWO
CSB-EP373613CWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides Heat-inducible transcription repressor HrcA (hrcA) CSB-YP373614CWO
CSB-EP373614CWO
CSB-BP373614CWO
CSB-MP373614CWO
CSB-EP373614CWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides DNA mismatch repair protein MutS (mutS), partial CSB-YP373615CWO
CSB-EP373615CWO
CSB-BP373615CWO
CSB-MP373615CWO
CSB-EP373615CWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP373616CWO
CSB-EP373616CWO
CSB-BP373616CWO
CSB-MP373616CWO
CSB-EP373616CWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides 60 kDa chaperonin (groL), partial CSB-YP373617CWO
CSB-EP373617CWO
CSB-BP373617CWO
CSB-MP373617CWO
CSB-EP373617CWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides 30S ribosomal protein S2 (rpsB) CSB-YP373618CWO
CSB-EP373618CWO
CSB-BP373618CWO
CSB-MP373618CWO
CSB-EP373618CWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides 5'-nucleotidase surE (surE) CSB-YP373619CWO
CSB-EP373619CWO
CSB-BP373619CWO
CSB-MP373619CWO
CSB-EP373619CWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB) CSB-YP373620CWO
CSB-EP373620CWO
CSB-BP373620CWO
CSB-MP373620CWO
CSB-EP373620CWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD), partial CSB-YP373621CWO
CSB-EP373621CWO
CSB-BP373621CWO
CSB-MP373621CWO
CSB-EP373621CWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP373622CWO
CSB-EP373622CWO
CSB-BP373622CWO
CSB-MP373622CWO
CSB-EP373622CWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides ATP phosphoribosyltransferase (hisG) CSB-YP373623CWO
CSB-EP373623CWO
CSB-BP373623CWO
CSB-MP373623CWO
CSB-EP373623CWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides Light-independent protochlorophyllide reductase iron-sulfur ATP-binding protein (bchL) CSB-YP373624CWO
CSB-EP373624CWO
CSB-BP373624CWO
CSB-MP373624CWO
CSB-EP373624CWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides 50S ribosomal protein L17 (rplQ) CSB-YP373625CWO
CSB-EP373625CWO
CSB-BP373625CWO
CSB-MP373625CWO
CSB-EP373625CWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides 30S ribosomal protein S11 (rpsK) CSB-YP373626CWO
CSB-EP373626CWO
CSB-BP373626CWO
CSB-MP373626CWO
CSB-EP373626CWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides Translation initiation factor IF-1 (infA) CSB-YP373627CWO
CSB-EP373627CWO
CSB-BP373627CWO
CSB-MP373627CWO
CSB-EP373627CWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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