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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Paracoccus denitrificans ATP synthase gamma chain (atpG) CSB-YP373568PYC
CSB-EP373568PYC
CSB-BP373568PYC
CSB-MP373568PYC
CSB-EP373568PYC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccus denitrificans Ribosome maturation factor RimM (rimM) CSB-YP373569PYC
CSB-EP373569PYC
CSB-BP373569PYC
CSB-MP373569PYC
CSB-EP373569PYC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccus denitrificans UPF0145 protein Pden_3965 (Pden_3965) CSB-YP373570PYC
CSB-EP373570PYC
CSB-BP373570PYC
CSB-MP373570PYC
CSB-EP373570PYC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccus denitrificans Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP373571PYC
CSB-EP373571PYC
CSB-BP373571PYC
CSB-MP373571PYC
CSB-EP373571PYC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccus denitrificans 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr) CSB-YP373572PYC
CSB-EP373572PYC
CSB-BP373572PYC
CSB-MP373572PYC
CSB-EP373572PYC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccus denitrificans Cysteine--tRNA ligase (cysS) CSB-YP373573PYC
CSB-EP373573PYC
CSB-BP373573PYC
CSB-MP373573PYC
CSB-EP373573PYC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccus denitrificans DNA ligase (ligA), partial CSB-YP373574PYC
CSB-EP373574PYC
CSB-BP373574PYC
CSB-MP373574PYC
CSB-EP373574PYC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccus denitrificans Aliphatic sulfonates import ATP-binding protein SsuB 1 (ssuB1) CSB-YP373575PYC
CSB-EP373575PYC
CSB-BP373575PYC
CSB-MP373575PYC
CSB-EP373575PYC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccus denitrificans 7-cyano-7-deazaguanine synthase (queC) CSB-YP373576PYC
CSB-EP373576PYC
CSB-BP373576PYC
CSB-MP373576PYC
CSB-EP373576PYC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccus denitrificans 30S ribosomal protein S4 (rpsD) CSB-YP373577PYC
CSB-EP373577PYC
CSB-BP373577PYC
CSB-MP373577PYC
CSB-EP373577PYC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccus denitrificans Protein nrdI (nrdI) CSB-YP373578PYC
CSB-EP373578PYC
CSB-BP373578PYC
CSB-MP373578PYC
CSB-EP373578PYC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccus denitrificans UDP-N-acetylenolpyruvoylglucosamine reductase (murB) CSB-YP373579PYC
CSB-EP373579PYC
CSB-BP373579PYC
CSB-MP373579PYC
CSB-EP373579PYC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccus denitrificans Gamma-glutamyl phosphate reductase (proA) CSB-YP373580PYC
CSB-EP373580PYC
CSB-BP373580PYC
CSB-MP373580PYC
CSB-EP373580PYC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccus denitrificans Flavoprotein wrbA (wrbA) CSB-YP373581PYC
CSB-EP373581PYC
CSB-BP373581PYC
CSB-MP373581PYC
CSB-EP373581PYC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccus denitrificans Bifunctional protein FolD 2 (folD2) CSB-YP373582PYC
CSB-EP373582PYC
CSB-BP373582PYC
CSB-MP373582PYC
CSB-EP373582PYC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides UPF0102 protein Cpha266_0037 (Cpha266_0037) CSB-YP373583CWO
CSB-EP373583CWO
CSB-BP373583CWO
CSB-MP373583CWO
CSB-EP373583CWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides tRNA modification GTPase MnmE (mnmE) CSB-YP373584CWO
CSB-EP373584CWO
CSB-BP373584CWO
CSB-MP373584CWO
CSB-EP373584CWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides 50S ribosomal protein L11 (rplK) CSB-YP373585CWO
CSB-EP373585CWO
CSB-BP373585CWO
CSB-MP373585CWO
CSB-EP373585CWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP373586CWO
CSB-EP373586CWO
CSB-BP373586CWO
CSB-MP373586CWO
CSB-EP373586CWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides 30S ribosomal protein S20 (rpsT) CSB-YP373587CWO
CSB-EP373587CWO
CSB-BP373587CWO
CSB-MP373587CWO
CSB-EP373587CWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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