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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B ATPase ravA (ravA), partial CSB-YP374168YAK
CSB-EP374168YAK
CSB-BP374168YAK
CSB-MP374168YAK
CSB-EP374168YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Phosphopantetheine adenylyltransferase (coaD) CSB-YP374169YAK
CSB-EP374169YAK
CSB-BP374169YAK
CSB-MP374169YAK
CSB-EP374169YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B D-tyrosyl-tRNA (Tyr) deacylase CSB-YP374170YAK
CSB-EP374170YAK
CSB-BP374170YAK
CSB-MP374170YAK
CSB-EP374170YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Ribonuclease PH (rph) CSB-YP374171YAK
CSB-EP374171YAK
CSB-BP374171YAK
CSB-MP374171YAK
CSB-EP374171YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Nucleoid occlusion factor SlmA (slmA) CSB-YP374172YAK
CSB-EP374172YAK
CSB-BP374172YAK
CSB-MP374172YAK
CSB-EP374172YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B 50S ribosomal protein L31 (rpmE) CSB-YP374173YAK
CSB-EP374173YAK
CSB-BP374173YAK
CSB-MP374173YAK
CSB-EP374173YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Soluble pyridine nucleotide transhydrogenase (sthA) CSB-YP374174YAK
CSB-EP374174YAK
CSB-BP374174YAK
CSB-MP374174YAK
CSB-EP374174YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B HTH-type transcriptional regulator hdfR (hdfR) CSB-YP374175YAK
CSB-EP374175YAK
CSB-BP374175YAK
CSB-MP374175YAK
CSB-EP374175YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase (gppA) CSB-YP374176YAK
CSB-EP374176YAK
CSB-BP374176YAK
CSB-MP374176YAK
CSB-EP374176YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Diaminopimelate epimerase (dapF) CSB-YP374177YAK
CSB-EP374177YAK
CSB-BP374177YAK
CSB-MP374177YAK
CSB-EP374177YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B sn-glycerol-3-phosphate transport system permease protein ugpE (ugpE), partial CSB-YP374178YAK1
CSB-EP374178YAK1
CSB-BP374178YAK1
CSB-MP374178YAK1
CSB-EP374178YAK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Probable ubiquinone biosynthesis protein UbiB (ubiB), partial CSB-YP374179YAK1
CSB-EP374179YAK1
CSB-BP374179YAK1
CSB-MP374179YAK1
CSB-EP374179YAK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Pantothenate kinase (coaA) CSB-YP374180YAK
CSB-EP374180YAK
CSB-BP374180YAK
CSB-MP374180YAK
CSB-EP374180YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B 50S ribosomal protein L10 (rplJ) CSB-YP374181YAK
CSB-EP374181YAK
CSB-BP374181YAK
CSB-MP374181YAK
CSB-EP374181YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B NADH pyrophosphatase (nudC) CSB-YP374182YAK
CSB-EP374182YAK
CSB-BP374182YAK
CSB-MP374182YAK
CSB-EP374182YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP374183YAK
CSB-EP374183YAK
CSB-BP374183YAK
CSB-MP374183YAK
CSB-EP374183YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Cation/acetate symporter ActP (actP), partial CSB-YP374184YAK
CSB-EP374184YAK
CSB-BP374184YAK
CSB-MP374184YAK
CSB-EP374184YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Thiol:disulfide interchange protein DsbD (dsbD), partial CSB-YP374185YAK
CSB-EP374185YAK
CSB-BP374185YAK
CSB-MP374185YAK
CSB-EP374185YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Uncharacterized protein YjeA (yjeA) CSB-YP374186YAK
CSB-EP374186YAK
CSB-BP374186YAK
CSB-MP374186YAK
CSB-EP374186YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Putative ribosome biogenesis GTPase RsgA (rsgA) CSB-YP374187YAK
CSB-EP374187YAK
CSB-BP374187YAK
CSB-MP374187YAK
CSB-EP374187YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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