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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Mycobacterium bovis Cobyric acid synthase (cobQ) CSB-YP375769MOJ
CSB-EP375769MOJ
CSB-BP375769MOJ
CSB-MP375769MOJ
CSB-EP375769MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis Adenylosuccinate synthetase (purA) CSB-YP375770MOJ
CSB-EP375770MOJ
CSB-BP375770MOJ
CSB-MP375770MOJ
CSB-EP375770MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis Phosphomethylpyrimidine synthase (thiC), partial CSB-YP375771MOJ
CSB-EP375771MOJ
CSB-BP375771MOJ
CSB-MP375771MOJ
CSB-EP375771MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis Heparin-binding hemagglutinin (hbhA) CSB-YP375772MOJ
CSB-EP375772MOJ
CSB-BP375772MOJ
CSB-MP375772MOJ
CSB-EP375772MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP375773MOJ
CSB-EP375773MOJ
CSB-BP375773MOJ
CSB-MP375773MOJ
CSB-EP375773MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis 3-oxoacyl-[acyl-carrier-protein] synthase 3 (fabH) CSB-YP375774MOJ
CSB-EP375774MOJ
CSB-BP375774MOJ
CSB-MP375774MOJ
CSB-EP375774MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis Probable endonuclease 4 (nfo) CSB-YP375775MOJ
CSB-EP375775MOJ
CSB-BP375775MOJ
CSB-MP375775MOJ
CSB-EP375775MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis 30S ribosomal protein S10 (rpsJ) CSB-YP375776MOJ
CSB-EP375776MOJ
CSB-BP375776MOJ
CSB-MP375776MOJ
CSB-EP375776MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis 50S ribosomal protein L23 (rplW) CSB-YP375777MOJ
CSB-EP375777MOJ
CSB-BP375777MOJ
CSB-MP375777MOJ
CSB-EP375777MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis 30S ribosomal protein S3 (rpsC) CSB-YP375778MOJ
CSB-EP375778MOJ
CSB-BP375778MOJ
CSB-MP375778MOJ
CSB-EP375778MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis 50S ribosomal protein L14 (rplN) CSB-YP375779MOJ
CSB-EP375779MOJ
CSB-BP375779MOJ
CSB-MP375779MOJ
CSB-EP375779MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis 30S ribosomal protein S8 (rpsH) CSB-YP375780MOJ
CSB-EP375780MOJ
CSB-BP375780MOJ
CSB-MP375780MOJ
CSB-EP375780MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis 30S ribosomal protein S5 (rpsE) CSB-YP375781MOJ
CSB-EP375781MOJ
CSB-BP375781MOJ
CSB-MP375781MOJ
CSB-EP375781MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis Putative S-adenosyl-L-methionine-dependent methyltransferase BCG_0776c (BCG_0776c) CSB-YP375782MOJ
CSB-EP375782MOJ
CSB-BP375782MOJ
CSB-MP375782MOJ
CSB-EP375782MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis Probable M18 family aminopeptidase 2 (apeB) CSB-YP375783MOJ
CSB-EP375783MOJ
CSB-BP375783MOJ
CSB-MP375783MOJ
CSB-EP375783MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis Putative S-adenosyl-L-methionine-dependent methyltransferase BCG_0883 (BCG_0883) CSB-YP375784MOJ
CSB-EP375784MOJ
CSB-BP375784MOJ
CSB-MP375784MOJ
CSB-EP375784MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis Putative S-adenosyl-L-methionine-dependent methyltransferase BCG_0945c (BCG_0945c) CSB-YP375785MOJ
CSB-EP375785MOJ
CSB-BP375785MOJ
CSB-MP375785MOJ
CSB-EP375785MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis Response regulator mprA (mprA) CSB-YP375786MOJ
CSB-EP375786MOJ
CSB-BP375786MOJ
CSB-MP375786MOJ
CSB-EP375786MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP375787MOJ
CSB-EP375787MOJ
CSB-BP375787MOJ
CSB-MP375787MOJ
CSB-EP375787MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis Potassium-transporting ATPase C chain (kdpC), partial CSB-YP375788MOJ
CSB-EP375788MOJ
CSB-BP375788MOJ
CSB-MP375788MOJ
CSB-EP375788MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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