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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP374649YAK
CSB-EP374649YAK
CSB-BP374649YAK
CSB-MP374649YAK
CSB-EP374649YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Protein translocase subunit SecA (secA), partial CSB-YP374650YAK
CSB-EP374650YAK
CSB-BP374650YAK
CSB-MP374650YAK
CSB-EP374650YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Pantothenate synthetase (panC) CSB-YP374651YAK
CSB-EP374651YAK
CSB-BP374651YAK
CSB-MP374651YAK
CSB-EP374651YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Sugar fermentation stimulation protein homolog (sfsA) CSB-YP374652YAK
CSB-EP374652YAK
CSB-BP374652YAK
CSB-MP374652YAK
CSB-EP374652YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B 23S rRNA (uracil (1939)-C (5))-methyltransferase RlmD CSB-YP374653YAK
CSB-EP374653YAK
CSB-BP374653YAK
CSB-MP374653YAK
CSB-EP374653YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Sulfite reductase [NADPH] flavoprotein alpha-component (cysJ), partial CSB-YP374654YAK
CSB-EP374654YAK
CSB-BP374654YAK
CSB-MP374654YAK
CSB-EP374654YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Phosphoadenosine phosphosulfate reductase (cysH) CSB-YP374655YAK
CSB-EP374655YAK
CSB-BP374655YAK
CSB-MP374655YAK
CSB-EP374655YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B 5'/3'-nucleotidase SurE (surE) CSB-YP374656YAK
CSB-EP374656YAK
CSB-BP374656YAK
CSB-MP374656YAK
CSB-EP374656YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B D-serine dehydratase (dsdA) CSB-YP374657YAK
CSB-EP374657YAK
CSB-BP374657YAK
CSB-MP374657YAK
CSB-EP374657YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B UPF0303 protein YE1367 (YE1367) CSB-YP374658YAK
CSB-EP374658YAK
CSB-BP374658YAK
CSB-MP374658YAK
CSB-EP374658YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Carbon storage regulator homolog (csrA) CSB-YP374659YAK
CSB-EP374659YAK
CSB-BP374659YAK
CSB-MP374659YAK
CSB-EP374659YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B tRNA (guanine-N (1)-)-methyltransferase CSB-YP374660YAK
CSB-EP374660YAK
CSB-BP374660YAK
CSB-MP374660YAK
CSB-EP374660YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B UPF0115 protein YE1279 (YE1279) CSB-YP374661YAK
CSB-EP374661YAK
CSB-BP374661YAK
CSB-MP374661YAK
CSB-EP374661YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B UPF0294 protein YE0917 (YE0917) CSB-YP374662YAK
CSB-EP374662YAK
CSB-BP374662YAK
CSB-MP374662YAK
CSB-EP374662YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Protein nrdI (nrdI) CSB-YP374663YAK
CSB-EP374663YAK
CSB-BP374663YAK
CSB-MP374663YAK
CSB-EP374663YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Urease accessory protein UreE (ureE) CSB-YP374664YAK
CSB-EP374664YAK
CSB-BP374664YAK
CSB-MP374664YAK
CSB-EP374664YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B UPF0249 protein YE0972 (YE0972) CSB-YP374665YAK
CSB-EP374665YAK
CSB-BP374665YAK
CSB-MP374665YAK
CSB-EP374665YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC (mnmC), partial CSB-YP374666YAK
CSB-EP374666YAK
CSB-BP374666YAK
CSB-MP374666YAK
CSB-EP374666YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B HTH-type transcriptional regulator iscR (iscR) CSB-YP374667YAK
CSB-EP374667YAK
CSB-BP374667YAK
CSB-MP374667YAK
CSB-EP374667YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Chaperone protein hscA (hscA), partial CSB-YP374668YAK
CSB-EP374668YAK
CSB-BP374668YAK
CSB-MP374668YAK
CSB-EP374668YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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