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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA) CSB-YP374709YAK
CSB-EP374709YAK
CSB-BP374709YAK
CSB-MP374709YAK
CSB-EP374709YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr) CSB-YP374710YAK
CSB-EP374710YAK
CSB-BP374710YAK
CSB-MP374710YAK
CSB-EP374710YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B 30S ribosomal protein S2 (rpsB) CSB-YP374711YAK
CSB-EP374711YAK
CSB-BP374711YAK
CSB-MP374711YAK
CSB-EP374711YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Protein syd (syd) CSB-YP374712YAK
CSB-EP374712YAK
CSB-BP374712YAK
CSB-MP374712YAK
CSB-EP374712YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Ribosomal RNA large subunit methyltransferase M (rlmM) CSB-YP374713YAK
CSB-EP374713YAK
CSB-BP374713YAK
CSB-MP374713YAK
CSB-EP374713YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Bifunctional protein aas (aas), partial CSB-YP374714YAK1
CSB-EP374714YAK1
CSB-BP374714YAK1
CSB-MP374714YAK1
CSB-EP374714YAK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Peptide chain release factor 2 (prfB) CSB-YP374715YAK
CSB-EP374715YAK
CSB-BP374715YAK
CSB-MP374715YAK
CSB-EP374715YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B UPF0149 protein YE3397 (YE3397) CSB-YP374716YAK
CSB-EP374716YAK
CSB-BP374716YAK
CSB-MP374716YAK
CSB-EP374716YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Ribose-5-phosphate isomerase A (rpiA) CSB-YP374717YAK
CSB-EP374717YAK
CSB-BP374717YAK
CSB-MP374717YAK
CSB-EP374717YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Phosphoglycerate kinase (pgk) CSB-YP374718YAK
CSB-EP374718YAK
CSB-BP374718YAK
CSB-MP374718YAK
CSB-EP374718YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B UPF0250 protein YE3006 (YE3006) CSB-YP374719YAK
CSB-EP374719YAK
CSB-BP374719YAK
CSB-MP374719YAK
CSB-EP374719YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Protein sprT (sprT) CSB-YP374720YAK
CSB-EP374720YAK
CSB-BP374720YAK
CSB-MP374720YAK
CSB-EP374720YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP374721YAK
CSB-EP374721YAK
CSB-BP374721YAK
CSB-MP374721YAK
CSB-EP374721YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B UPF0259 membrane protein YE2218 (YE2218), partial CSB-YP374722YAK1
CSB-EP374722YAK1
CSB-BP374722YAK1
CSB-MP374722YAK1
CSB-EP374722YAK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP374723YAK
CSB-EP374723YAK
CSB-BP374723YAK
CSB-MP374723YAK
CSB-EP374723YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Hydroxyethylthiazole kinase (thiM) CSB-YP374724YAK
CSB-EP374724YAK
CSB-BP374724YAK
CSB-MP374724YAK
CSB-EP374724YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Na (+)/H (+) antiporter nhaB, partial CSB-YP374725YAK
CSB-EP374725YAK
CSB-BP374725YAK
CSB-MP374725YAK
CSB-EP374725YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Disulfide bond formation protein B (dsbB), partial CSB-YP374726YAK1
CSB-EP374726YAK1
CSB-BP374726YAK1
CSB-MP374726YAK1
CSB-EP374726YAK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP374727YAK1
CSB-EP374727YAK1
CSB-BP374727YAK1
CSB-MP374727YAK1
CSB-EP374727YAK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Potassium-transporting ATPase A chain (kdpA), partial CSB-YP374728YAK
CSB-EP374728YAK
CSB-BP374728YAK
CSB-MP374728YAK
CSB-EP374728YAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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