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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Mycobacterium bovis Proline--tRNA ligase (proS), partial CSB-YP374929MOJ
CSB-EP374929MOJ
CSB-BP374929MOJ
CSB-MP374929MOJ
CSB-EP374929MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis UPF0102 protein BCG_2919c (BCG_2919c) CSB-YP374930MOJ
CSB-EP374930MOJ
CSB-BP374930MOJ
CSB-MP374930MOJ
CSB-EP374930MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis 50S ribosomal protein L19 (rplS) CSB-YP374931MOJ
CSB-EP374931MOJ
CSB-BP374931MOJ
CSB-MP374931MOJ
CSB-EP374931MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis 30S ribosomal protein S16 (rpsP) CSB-YP374932MOJ
CSB-EP374932MOJ
CSB-BP374932MOJ
CSB-MP374932MOJ
CSB-EP374932MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis Phthiodiolone/phenolphthiodiolone dimycocerosates ketoreductase (BCG_2972c) CSB-YP374933MOJ
CSB-EP374933MOJ
CSB-BP374933MOJ
CSB-MP374933MOJ
CSB-EP374933MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis Inactive PGL/p-HBAD biosynthesis glycosyltransferase BCG_2979c (BCG_2979c) CSB-YP374934MOJ
CSB-EP374934MOJ
CSB-BP374934MOJ
CSB-MP374934MOJ
CSB-EP374934MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis D-alanine--D-alanine ligase (ddl) CSB-YP374935MOJ
CSB-EP374935MOJ
CSB-BP374935MOJ
CSB-MP374935MOJ
CSB-EP374935MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP374936MOJ
CSB-EP374936MOJ
CSB-BP374936MOJ
CSB-MP374936MOJ
CSB-EP374936MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis Glutamate--tRNA ligase (gltX) CSB-YP374937MOJ
CSB-EP374937MOJ
CSB-BP374937MOJ
CSB-MP374937MOJ
CSB-EP374937MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B, partial CSB-YP374938MOJ
CSB-EP374938MOJ
CSB-BP374938MOJ
CSB-MP374938MOJ
CSB-EP374938MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis Glutamyl-tRNA (Gln) amidotransferase subunit A CSB-YP374939MOJ
CSB-EP374939MOJ
CSB-BP374939MOJ
CSB-MP374939MOJ
CSB-EP374939MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C CSB-YP374940MOJ
CSB-EP374940MOJ
CSB-BP374940MOJ
CSB-MP374940MOJ
CSB-EP374940MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis Probable enoyl-CoA hydratase echA17 (echA17) CSB-YP374941MOJ
CSB-EP374941MOJ
CSB-BP374941MOJ
CSB-MP374941MOJ
CSB-EP374941MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis Transcriptional regulatory protein MoaR1 (moaR1) CSB-YP374942MOJ
CSB-EP374942MOJ
CSB-BP374942MOJ
CSB-MP374942MOJ
CSB-EP374942MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP374943MOJ
CSB-EP374943MOJ
CSB-BP374943MOJ
CSB-MP374943MOJ
CSB-EP374943MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis NADH-quinone oxidoreductase subunit I (nuoI) CSB-YP374944MOJ
CSB-EP374944MOJ
CSB-BP374944MOJ
CSB-MP374944MOJ
CSB-EP374944MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis Protein translocase subunit SecA 1 (secA1), partial CSB-YP374945MOJ
CSB-EP374945MOJ
CSB-BP374945MOJ
CSB-MP374945MOJ
CSB-EP374945MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis Pilin (mtp) CSB-YP374946MOJ
CSB-EP374946MOJ
CSB-BP374946MOJ
CSB-MP374946MOJ
CSB-EP374946MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis Bifunctional protein FolD (folD) CSB-YP374947MOJ
CSB-EP374947MOJ
CSB-BP374947MOJ
CSB-MP374947MOJ
CSB-EP374947MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis 60 kDa chaperonin 2 (groL2), partial CSB-YP374948MOJ
CSB-EP374948MOJ
CSB-BP374948MOJ
CSB-MP374948MOJ
CSB-EP374948MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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