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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Mycobacterium bovis 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP374889MOJ
CSB-EP374889MOJ
CSB-BP374889MOJ
CSB-MP374889MOJ
CSB-EP374889MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis Peptidyl-tRNA hydrolase (pth) CSB-YP374890MOJ
CSB-EP374890MOJ
CSB-BP374890MOJ
CSB-MP374890MOJ
CSB-EP374890MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis Enolase (eno) CSB-YP374891MOJ
CSB-EP374891MOJ
CSB-BP374891MOJ
CSB-MP374891MOJ
CSB-EP374891MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis Transcription elongation factor GreA (greA) CSB-YP374892MOJ
CSB-EP374892MOJ
CSB-BP374892MOJ
CSB-MP374892MOJ
CSB-EP374892MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE), partial CSB-YP374893MOJ
CSB-EP374893MOJ
CSB-BP374893MOJ
CSB-MP374893MOJ
CSB-EP374893MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis 1D-myo-inositol 2-acetamido-2-deoxy-alpha-D-glucopyranoside deacetylase (mshB) CSB-YP374894MOJ
CSB-EP374894MOJ
CSB-BP374894MOJ
CSB-MP374894MOJ
CSB-EP374894MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis ATP synthase gamma chain (atpG) CSB-YP374895MOJ
CSB-EP374895MOJ
CSB-BP374895MOJ
CSB-MP374895MOJ
CSB-EP374895MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis ATP-dependent Clp protease adapter protein ClpS (clpS) CSB-YP374896MOJ
CSB-EP374896MOJ
CSB-BP374896MOJ
CSB-MP374896MOJ
CSB-EP374896MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis Bifunctional protein pyrR (pyrR) CSB-YP374897MOJ
CSB-EP374897MOJ
CSB-BP374897MOJ
CSB-MP374897MOJ
CSB-EP374897MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis S-adenosylmethionine synthase (metK) CSB-YP374898MOJ
CSB-EP374898MOJ
CSB-BP374898MOJ
CSB-MP374898MOJ
CSB-EP374898MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis UvrABC system protein C (uvrC), partial CSB-YP374899MOJ
CSB-EP374899MOJ
CSB-BP374899MOJ
CSB-MP374899MOJ
CSB-EP374899MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis Quinolinate synthase A (nadA) CSB-YP374900MOJ
CSB-EP374900MOJ
CSB-BP374900MOJ
CSB-MP374900MOJ
CSB-EP374900MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis Indole-3-glycerol phosphate synthase (trpC) CSB-YP374901MOJ
CSB-EP374901MOJ
CSB-BP374901MOJ
CSB-MP374901MOJ
CSB-EP374901MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis 50S ribosomal protein L35 (rpmI) CSB-YP374902MOJ
CSB-EP374902MOJ
CSB-BP374902MOJ
CSB-MP374902MOJ
CSB-EP374902MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP374903MOJ
CSB-EP374903MOJ
CSB-BP374903MOJ
CSB-MP374903MOJ
CSB-EP374903MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis Protein translocase subunit SecA 2 (secA2), partial CSB-YP374904MOJ
CSB-EP374904MOJ
CSB-BP374904MOJ
CSB-MP374904MOJ
CSB-EP374904MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis Malate synthase G (glcB), partial CSB-YP374905MOJ
CSB-EP374905MOJ
CSB-BP374905MOJ
CSB-MP374905MOJ
CSB-EP374905MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis Urease accessory protein UreF (ureF) CSB-YP374906MOJ
CSB-EP374906MOJ
CSB-BP374906MOJ
CSB-MP374906MOJ
CSB-EP374906MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis D-tyrosyl-tRNA (Tyr) deacylase CSB-YP374907MOJ
CSB-EP374907MOJ
CSB-BP374907MOJ
CSB-MP374907MOJ
CSB-EP374907MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis 30S ribosomal protein S18 2 (rpsR2) CSB-YP374908MOJ
CSB-EP374908MOJ
CSB-BP374908MOJ
CSB-MP374908MOJ
CSB-EP374908MOJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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