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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Cobyric acid synthase (cobQ), partial CSB-YP375229YAK
CSB-EP375229YAK
CSB-BP375229YAK
CSB-MP375229YAK
CSB-EP375229YAK-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP375230YAK
CSB-EP375230YAK
CSB-BP375230YAK
CSB-MP375230YAK
CSB-EP375230YAK-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Cysteine protease yopT1 (yopT1) CSB-YP375231YAK
CSB-EP375231YAK
CSB-BP375231YAK
CSB-MP375231YAK
CSB-EP375231YAK-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Chaperone protein sycN (sycN) CSB-YP375232YAK
CSB-EP375232YAK
CSB-BP375232YAK
CSB-MP375232YAK
CSB-EP375232YAK-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia enterocolitica serotype O:8 / biotype 1B Antitoxin ParD (parD) CSB-YP375233YAK
CSB-EP375233YAK
CSB-BP375233YAK
CSB-MP375233YAK
CSB-EP375233YAK-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. UPF0271 protein azo0043 (azo0043) CSB-YP375234AVL
CSB-EP375234AVL
CSB-BP375234AVL
CSB-MP375234AVL
CSB-EP375234AVL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP375235AVL
CSB-EP375235AVL
CSB-BP375235AVL
CSB-MP375235AVL
CSB-EP375235AVL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. ATP synthase subunit c (atpE), partial CSB-YP375236AVL1
CSB-EP375236AVL1
CSB-BP375236AVL1
CSB-MP375236AVL1
CSB-EP375236AVL1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. ATP synthase epsilon chain (atpC) CSB-YP375237AVL
CSB-EP375237AVL
CSB-BP375237AVL
CSB-MP375237AVL
CSB-EP375237AVL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. Glutamyl-tRNA (Gln) amidotransferase subunit A CSB-YP375238AVL
CSB-EP375238AVL
CSB-BP375238AVL
CSB-MP375238AVL
CSB-EP375238AVL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. Arginine--tRNA ligase (argS), partial CSB-YP375239AVL
CSB-EP375239AVL
CSB-BP375239AVL
CSB-MP375239AVL
CSB-EP375239AVL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. Probable chorismate--pyruvate lyase (ubiC) CSB-YP375240AVL
CSB-EP375240AVL
CSB-BP375240AVL
CSB-MP375240AVL
CSB-EP375240AVL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. Multifunctional CCA protein (cca) CSB-YP375241AVL
CSB-EP375241AVL
CSB-BP375241AVL
CSB-MP375241AVL
CSB-EP375241AVL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. Electron transport complex protein RnfA (rnfA), partial CSB-YP375242AVL1
CSB-EP375242AVL1
CSB-BP375242AVL1
CSB-MP375242AVL1
CSB-EP375242AVL1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP375243AVL
CSB-EP375243AVL
CSB-BP375243AVL
CSB-MP375243AVL
CSB-EP375243AVL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. UPF0502 protein azo0627 (azo0627) CSB-YP375244AVL
CSB-EP375244AVL
CSB-BP375244AVL
CSB-MP375244AVL
CSB-EP375244AVL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. Shikimate dehydrogenase (aroE) CSB-YP375245AVL
CSB-EP375245AVL
CSB-BP375245AVL
CSB-MP375245AVL
CSB-EP375245AVL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. 50S ribosomal protein L9 (rplI) CSB-YP375246AVL
CSB-EP375246AVL
CSB-BP375246AVL
CSB-MP375246AVL
CSB-EP375246AVL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. Type III pantothenate kinase (coaX) CSB-YP375247AVL
CSB-EP375247AVL
CSB-BP375247AVL
CSB-MP375247AVL
CSB-EP375247AVL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azoarcus sp. 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP375248AVL
CSB-EP375248AVL
CSB-BP375248AVL
CSB-MP375248AVL
CSB-EP375248AVL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達(dá)系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子，它攜帶特定的基因進(jìn)入宿主細(xì)胞，并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá)，經(jīng)過多年的發(fā)展，表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示，本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條：如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用，對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達(dá)服務(wù)，您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等，您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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