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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Shewanella sp. Chaperone protein DnaJ (dnaJ) CSB-YP376829STT
CSB-EP376829STT
CSB-BP376829STT
CSB-MP376829STT
CSB-EP376829STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Ribosomal RNA large subunit methyltransferase I (rlmI) CSB-YP376830STT
CSB-EP376830STT
CSB-BP376830STT
CSB-MP376830STT
CSB-EP376830STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP376831STT
CSB-EP376831STT
CSB-BP376831STT
CSB-MP376831STT
CSB-EP376831STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Leucine--tRNA ligase (leuS), partial CSB-YP376832STT
CSB-EP376832STT
CSB-BP376832STT
CSB-MP376832STT
CSB-EP376832STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP376833STT
CSB-EP376833STT
CSB-BP376833STT
CSB-MP376833STT
CSB-EP376833STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Ribosome-binding factor A (rbfA) CSB-YP376834STT
CSB-EP376834STT
CSB-BP376834STT
CSB-MP376834STT
CSB-EP376834STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. UPF0060 membrane protein Sputw3181_1172 (Sputw3181_1172), partial CSB-YP376835STT1
CSB-EP376835STT1
CSB-BP376835STT1
CSB-MP376835STT1
CSB-EP376835STT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Peptide chain release factor 3 (prfC), partial CSB-YP376836STT
CSB-EP376836STT
CSB-BP376836STT
CSB-MP376836STT
CSB-EP376836STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Phosphopentomutase (deoB) CSB-YP376837STT
CSB-EP376837STT
CSB-BP376837STT
CSB-MP376837STT
CSB-EP376837STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. N utilization substance protein B homolog (nusB) CSB-YP376838STT
CSB-EP376838STT
CSB-BP376838STT
CSB-MP376838STT
CSB-EP376838STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. 23S rRNA (uracil (1939)-C (5))-methyltransferase RlmD CSB-YP376839STT
CSB-EP376839STT
CSB-BP376839STT
CSB-MP376839STT
CSB-EP376839STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP376840STT
CSB-EP376840STT
CSB-BP376840STT
CSB-MP376840STT
CSB-EP376840STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. 5'-nucleotidase surE (surE) CSB-YP376841STT
CSB-EP376841STT
CSB-BP376841STT
CSB-MP376841STT
CSB-EP376841STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. UPF0312 protein Sputw3181_1309 (Sputw3181_1309) CSB-YP376842STT
CSB-EP376842STT
CSB-BP376842STT
CSB-MP376842STT
CSB-EP376842STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. GTPase Der (der) CSB-YP376843STT
CSB-EP376843STT
CSB-BP376843STT
CSB-MP376843STT
CSB-EP376843STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Proline--tRNA ligase (proS), partial CSB-YP376844STT
CSB-EP376844STT
CSB-BP376844STT
CSB-MP376844STT
CSB-EP376844STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Chorismate synthase (aroC) CSB-YP376845STT
CSB-EP376845STT
CSB-BP376845STT
CSB-MP376845STT
CSB-EP376845STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC (mnmC), partial CSB-YP376846STT
CSB-EP376846STT
CSB-BP376846STT
CSB-MP376846STT
CSB-EP376846STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. UPF0133 protein Sputw3181_1707 (Sputw3181_1707) CSB-YP376847STT
CSB-EP376847STT
CSB-BP376847STT
CSB-MP376847STT
CSB-EP376847STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. High frequency lysogenization protein HflD homolog (hflD) CSB-YP376848STT
CSB-EP376848STT
CSB-BP376848STT
CSB-MP376848STT
CSB-EP376848STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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