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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Nocardioides sp. 50S ribosomal protein L16 (rplP) CSB-YP377189NFC
CSB-EP377189NFC
CSB-BP377189NFC
CSB-MP377189NFC
CSB-EP377189NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. 30S ribosomal protein S19 (rpsS) CSB-YP377190NFC
CSB-EP377190NFC
CSB-BP377190NFC
CSB-MP377190NFC
CSB-EP377190NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. 50S ribosomal protein L2 (rplB) CSB-YP377191NFC
CSB-EP377191NFC
CSB-BP377191NFC
CSB-MP377191NFC
CSB-EP377191NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. tRNA (guanine-N (7)-)-methyltransferase CSB-YP377192NFC
CSB-EP377192NFC
CSB-BP377192NFC
CSB-MP377192NFC
CSB-EP377192NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. 30S ribosomal protein S12 (rpsL) CSB-YP377193NFC
CSB-EP377193NFC
CSB-BP377193NFC
CSB-MP377193NFC
CSB-EP377193NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. Cysteine--tRNA ligase (cysS) CSB-YP377194NFC
CSB-EP377194NFC
CSB-BP377194NFC
CSB-MP377194NFC
CSB-EP377194NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. D-inositol 3-phosphate glycosyltransferase (mshA) CSB-YP377195NFC
CSB-EP377195NFC
CSB-BP377195NFC
CSB-MP377195NFC
CSB-EP377195NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. Phosphoribosylformylglycinamidine synthase 2 (purL), partial CSB-YP377196NFC
CSB-EP377196NFC
CSB-BP377196NFC
CSB-MP377196NFC
CSB-EP377196NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. Orotate phosphoribosyltransferase (pyrE) CSB-YP377197NFC
CSB-EP377197NFC
CSB-BP377197NFC
CSB-MP377197NFC
CSB-EP377197NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. Deoxycytidine triphosphate deaminase (dcd) CSB-YP377198NFC
CSB-EP377198NFC
CSB-BP377198NFC
CSB-MP377198NFC
CSB-EP377198NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. 30S ribosomal protein S6 (rpsF) CSB-YP377199NFC
CSB-EP377199NFC
CSB-BP377199NFC
CSB-MP377199NFC
CSB-EP377199NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nocardioides sp. 50S ribosomal protein L34 (rpmH) CSB-YP377200NFC
CSB-EP377200NFC
CSB-BP377200NFC
CSB-MP377200NFC
CSB-EP377200NFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP377201PZS
CSB-EP377201PZS
CSB-BP377201PZS
CSB-MP377201PZS
CSB-EP377201PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii Tyrosine recombinase XerC (xerC) CSB-YP377202PZS
CSB-EP377202PZS
CSB-BP377202PZS
CSB-MP377202PZS
CSB-EP377202PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii Protein CyaY (cyaY) CSB-YP377203PZS
CSB-EP377203PZS
CSB-BP377203PZS
CSB-MP377203PZS
CSB-EP377203PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP377204PZS
CSB-EP377204PZS
CSB-BP377204PZS
CSB-MP377204PZS
CSB-EP377204PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii 50S ribosomal protein L28 (rpmB) CSB-YP377205PZS
CSB-EP377205PZS
CSB-BP377205PZS
CSB-MP377205PZS
CSB-EP377205PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii Shikimate dehydrogenase (aroE) CSB-YP377206PZS
CSB-EP377206PZS
CSB-BP377206PZS
CSB-MP377206PZS
CSB-EP377206PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii Mannitol-1-phosphate 5-dehydrogenase (mtlD) CSB-YP377207PZS
CSB-EP377207PZS
CSB-BP377207PZS
CSB-MP377207PZS
CSB-EP377207PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii Methylglyoxal synthase (mgsA) CSB-YP377208PZS
CSB-EP377208PZS
CSB-BP377208PZS
CSB-MP377208PZS
CSB-EP377208PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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