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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Psychromonas ingrahamii Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP377269PZS
CSB-EP377269PZS
CSB-BP377269PZS
CSB-MP377269PZS
CSB-EP377269PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii Asparagine--tRNA ligase (asnS) CSB-YP377270PZS
CSB-EP377270PZS
CSB-BP377270PZS
CSB-MP377270PZS
CSB-EP377270PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC (mnmC), partial CSB-YP377271PZS
CSB-EP377271PZS
CSB-BP377271PZS
CSB-MP377271PZS
CSB-EP377271PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii UDP-2,3-diacylglucosamine hydrolase (lpxH) CSB-YP377272PZS
CSB-EP377272PZS
CSB-BP377272PZS
CSB-MP377272PZS
CSB-EP377272PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii Threonine--tRNA ligase (thrS), partial CSB-YP377273PZS
CSB-EP377273PZS
CSB-BP377273PZS
CSB-MP377273PZS
CSB-EP377273PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii Recombination-associated protein rdgC (rdgC) CSB-YP377274PZS
CSB-EP377274PZS
CSB-BP377274PZS
CSB-MP377274PZS
CSB-EP377274PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP377275PZS
CSB-EP377275PZS
CSB-BP377275PZS
CSB-MP377275PZS
CSB-EP377275PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii UPF0234 protein Ping_2261 (Ping_2261) CSB-YP377276PZS
CSB-EP377276PZS
CSB-BP377276PZS
CSB-MP377276PZS
CSB-EP377276PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii UPF0133 protein Ping_2276 (Ping_2276) CSB-YP377277PZS
CSB-EP377277PZS
CSB-BP377277PZS
CSB-MP377277PZS
CSB-EP377277PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii Ribosomal RNA large subunit methyltransferase L (rlmL), partial CSB-YP377278PZS
CSB-EP377278PZS
CSB-BP377278PZS
CSB-MP377278PZS
CSB-EP377278PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii Dihydroorotate dehydrogenase (quinone) CSB-YP377279PZS
CSB-EP377279PZS
CSB-BP377279PZS
CSB-MP377279PZS
CSB-EP377279PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii Succinyl-diaminopimelate desuccinylase (dapE) CSB-YP377280PZS
CSB-EP377280PZS
CSB-BP377280PZS
CSB-MP377280PZS
CSB-EP377280PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii 30S ribosomal protein S11 (rpsK1) CSB-YP377281PZS
CSB-EP377281PZS
CSB-BP377281PZS
CSB-MP377281PZS
CSB-EP377281PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii Protein syd (syd) CSB-YP377282PZS
CSB-EP377282PZS
CSB-BP377282PZS
CSB-MP377282PZS
CSB-EP377282PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii Ribosomal protein S6 modification protein (rimK) CSB-YP377283PZS
CSB-EP377283PZS
CSB-BP377283PZS
CSB-MP377283PZS
CSB-EP377283PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii 50S ribosomal protein L31 (rpmE) CSB-YP377284PZS
CSB-EP377284PZS
CSB-BP377284PZS
CSB-MP377284PZS
CSB-EP377284PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii Purine nucleoside phosphorylase DeoD-type (deoD) CSB-YP377285PZS
CSB-EP377285PZS
CSB-BP377285PZS
CSB-MP377285PZS
CSB-EP377285PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii 50S ribosomal protein L13 (rplM) CSB-YP377286PZS
CSB-EP377286PZS
CSB-BP377286PZS
CSB-MP377286PZS
CSB-EP377286PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii Gamma-glutamyl phosphate reductase (proA) CSB-YP377287PZS
CSB-EP377287PZS
CSB-BP377287PZS
CSB-MP377287PZS
CSB-EP377287PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psychromonas ingrahamii (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP377288PZS
CSB-EP377288PZS
CSB-BP377288PZS
CSB-MP377288PZS
CSB-EP377288PZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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