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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Marinobacter aquaeolei Phosphoribosylglycinamide formyltransferase 2 (purT) CSB-YP378209MNI
CSB-EP378209MNI
CSB-BP378209MNI
CSB-MP378209MNI
CSB-EP378209MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Membrane-bound lytic murein transglycosylase F (mltF) CSB-YP378210MNI
CSB-EP378210MNI
CSB-BP378210MNI
CSB-MP378210MNI
CSB-EP378210MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Uridylate kinase (pyrH) CSB-YP378211MNI
CSB-EP378211MNI
CSB-BP378211MNI
CSB-MP378211MNI
CSB-EP378211MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Succinyl-diaminopimelate desuccinylase (dapE) CSB-YP378212MNI
CSB-EP378212MNI
CSB-BP378212MNI
CSB-MP378212MNI
CSB-EP378212MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Adenylyl-sulfate kinase (cysC) CSB-YP378213MNI
CSB-EP378213MNI
CSB-BP378213MNI
CSB-MP378213MNI
CSB-EP378213MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei ATP phosphoribosyltransferase (hisG) CSB-YP378214MNI
CSB-EP378214MNI
CSB-BP378214MNI
CSB-MP378214MNI
CSB-EP378214MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP378215MNI
CSB-EP378215MNI
CSB-BP378215MNI
CSB-MP378215MNI
CSB-EP378215MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C CSB-YP378216MNI
CSB-EP378216MNI
CSB-BP378216MNI
CSB-MP378216MNI
CSB-EP378216MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP378217MNI
CSB-EP378217MNI
CSB-BP378217MNI
CSB-MP378217MNI
CSB-EP378217MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Sulfate adenylyltransferase subunit 2 2 (cysD2) CSB-YP378218MNI
CSB-EP378218MNI
CSB-BP378218MNI
CSB-MP378218MNI
CSB-EP378218MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Probable rRNA maturation factor (Maqu_2740) CSB-YP378219MNI
CSB-EP378219MNI
CSB-BP378219MNI
CSB-MP378219MNI
CSB-EP378219MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei ATP-dependent dethiobiotin synthetase BioD (bioD) CSB-YP378220MNI
CSB-EP378220MNI
CSB-BP378220MNI
CSB-MP378220MNI
CSB-EP378220MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Biotin synthase (bioB) CSB-YP378221MNI
CSB-EP378221MNI
CSB-BP378221MNI
CSB-MP378221MNI
CSB-EP378221MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Adenylosuccinate synthetase (purA) CSB-YP378222MNI
CSB-EP378222MNI
CSB-BP378222MNI
CSB-MP378222MNI
CSB-EP378222MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Protein hfq (hfq) CSB-YP378223MNI
CSB-EP378223MNI
CSB-BP378223MNI
CSB-MP378223MNI
CSB-EP378223MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Putative ribosome biogenesis GTPase RsgA (rsgA) CSB-YP378224MNI
CSB-EP378224MNI
CSB-BP378224MNI
CSB-MP378224MNI
CSB-EP378224MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Protein-L-isoaspartate O-methyltransferase 2 (pcm2) CSB-YP378225MNI
CSB-EP378225MNI
CSB-BP378225MNI
CSB-MP378225MNI
CSB-EP378225MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Elongation factor P-like protein (Maqu_2909) CSB-YP378226MNI
CSB-EP378226MNI
CSB-BP378226MNI
CSB-MP378226MNI
CSB-EP378226MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Urease accessory protein UreE (ureE) CSB-YP378227MNI
CSB-EP378227MNI
CSB-BP378227MNI
CSB-MP378227MNI
CSB-EP378227MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Urease subunit gamma (ureA) CSB-YP378228MNI
CSB-EP378228MNI
CSB-BP378228MNI
CSB-MP378228MNI
CSB-EP378228MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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