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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Marinobacter aquaeolei Ribosomal RNA large subunit methyltransferase L (rlmL), partial CSB-YP378169MNI
CSB-EP378169MNI
CSB-BP378169MNI
CSB-MP378169MNI
CSB-EP378169MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP378170MNI
CSB-EP378170MNI
CSB-BP378170MNI
CSB-MP378170MNI
CSB-EP378170MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei DNA ligase (ligA), partial CSB-YP378171MNI
CSB-EP378171MNI
CSB-BP378171MNI
CSB-MP378171MNI
CSB-EP378171MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Macrolide export ATP-binding/permease protein MacB (macB), partial CSB-YP378172MNI1
CSB-EP378172MNI1
CSB-BP378172MNI1
CSB-MP378172MNI1
CSB-EP378172MNI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei 3-oxoacyl-[acyl-carrier-protein] synthase 3 (fabH) CSB-YP378173MNI
CSB-EP378173MNI
CSB-BP378173MNI
CSB-MP378173MNI
CSB-EP378173MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Tryptophan synthase alpha chain (trpA) CSB-YP378174MNI
CSB-EP378174MNI
CSB-BP378174MNI
CSB-MP378174MNI
CSB-EP378174MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP378175MNI
CSB-EP378175MNI
CSB-BP378175MNI
CSB-MP378175MNI
CSB-EP378175MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Chorismate synthase (aroC) CSB-YP378176MNI
CSB-EP378176MNI
CSB-BP378176MNI
CSB-MP378176MNI
CSB-EP378176MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP378177MNI
CSB-EP378177MNI
CSB-BP378177MNI
CSB-MP378177MNI
CSB-EP378177MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Dihydrodipicolinate synthase (dapA) CSB-YP378178MNI
CSB-EP378178MNI
CSB-BP378178MNI
CSB-MP378178MNI
CSB-EP378178MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP378179MNI
CSB-EP378179MNI
CSB-BP378179MNI
CSB-MP378179MNI
CSB-EP378179MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Putative arginyl-tRNA--protein transferase (ate) CSB-YP378180MNI
CSB-EP378180MNI
CSB-BP378180MNI
CSB-MP378180MNI
CSB-EP378180MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Translation initiation factor IF-1 (infA) CSB-YP378181MNI
CSB-EP378181MNI
CSB-BP378181MNI
CSB-MP378181MNI
CSB-EP378181MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei High frequency lysogenization protein HflD homolog (hflD) CSB-YP378182MNI
CSB-EP378182MNI
CSB-BP378182MNI
CSB-MP378182MNI
CSB-EP378182MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei tRNA 2-thiocytidine biosynthesis protein TtcA (ttcA) CSB-YP378183MNI
CSB-EP378183MNI
CSB-BP378183MNI
CSB-MP378183MNI
CSB-EP378183MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Bifunctional protein FolD (folD) CSB-YP378184MNI
CSB-EP378184MNI
CSB-BP378184MNI
CSB-MP378184MNI
CSB-EP378184MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Malate synthase G (glcB), partial CSB-YP378185MNI
CSB-EP378185MNI
CSB-BP378185MNI
CSB-MP378185MNI
CSB-EP378185MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Phosphate acyltransferase (plsX) CSB-YP378186MNI
CSB-EP378186MNI
CSB-BP378186MNI
CSB-MP378186MNI
CSB-EP378186MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Cytochrome c-type biogenesis protein CcmE (ccmE), partial CSB-YP378187MNI
CSB-EP378187MNI
CSB-BP378187MNI
CSB-MP378187MNI
CSB-EP378187MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Serine/threonine transporter sstT (sstT), partial CSB-YP378188MNI
CSB-EP378188MNI
CSB-BP378188MNI
CSB-MP378188MNI
CSB-EP378188MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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