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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Mycobacterium sp. 30S ribosomal protein S16 (rpsP) CSB-YP378309MOM
CSB-EP378309MOM
CSB-BP378309MOM
CSB-MP378309MOM
CSB-EP378309MOM-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Ribonuclease HII (rnhB) CSB-YP378310MOM
CSB-EP378310MOM
CSB-BP378310MOM
CSB-MP378310MOM
CSB-EP378310MOM-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Uridylate kinase (pyrH) CSB-YP378311MOM
CSB-EP378311MOM
CSB-BP378311MOM
CSB-MP378311MOM
CSB-EP378311MOM-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Cobyric acid synthase (cobQ) CSB-YP378312MOM
CSB-EP378312MOM
CSB-BP378312MOM
CSB-MP378312MOM
CSB-EP378312MOM-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Proline--tRNA ligase (proS), partial CSB-YP378313MOM
CSB-EP378313MOM
CSB-BP378313MOM
CSB-MP378313MOM
CSB-EP378313MOM-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Ribosome-binding factor A (rbfA) CSB-YP378314MOM
CSB-EP378314MOM
CSB-BP378314MOM
CSB-MP378314MOM
CSB-EP378314MOM-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. 30S ribosomal protein S15 (rpsO) CSB-YP378315MOM
CSB-EP378315MOM
CSB-BP378315MOM
CSB-MP378315MOM
CSB-EP378315MOM-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Dihydrodipicolinate reductase (dapB) CSB-YP378316MOM
CSB-EP378316MOM
CSB-BP378316MOM
CSB-MP378316MOM
CSB-EP378316MOM-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Protein RecA (recA) CSB-YP378317MOM
CSB-EP378317MOM
CSB-BP378317MOM
CSB-MP378317MOM
CSB-EP378317MOM-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Regulatory protein recX (recX) CSB-YP378318MOM
CSB-EP378318MOM
CSB-BP378318MOM
CSB-MP378318MOM
CSB-EP378318MOM-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Diaminopimelate epimerase (dapF) CSB-YP378319MOM
CSB-EP378319MOM
CSB-BP378319MOM
CSB-MP378319MOM
CSB-EP378319MOM-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut) CSB-YP378320MOM
CSB-EP378320MOM
CSB-BP378320MOM
CSB-MP378320MOM
CSB-EP378320MOM-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP378321MOM
CSB-EP378321MOM
CSB-BP378321MOM
CSB-MP378321MOM
CSB-EP378321MOM-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Threonine--tRNA ligase (thrS), partial CSB-YP378322MOM
CSB-EP378322MOM
CSB-BP378322MOM
CSB-MP378322MOM
CSB-EP378322MOM-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Probable transcriptional regulatory protein Mkms_2298 (Mkms_2298) CSB-YP378323MOM
CSB-EP378323MOM
CSB-BP378323MOM
CSB-MP378323MOM
CSB-EP378323MOM-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP378324MOM
CSB-EP378324MOM
CSB-BP378324MOM
CSB-MP378324MOM
CSB-EP378324MOM-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Aspartate--tRNA ligase (aspS), partial CSB-YP378325MOM
CSB-EP378325MOM
CSB-BP378325MOM
CSB-MP378325MOM
CSB-EP378325MOM-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Gas vesicle structural protein (gvpA) CSB-YP378326MOM
CSB-EP378326MOM
CSB-BP378326MOM
CSB-MP378326MOM
CSB-EP378326MOM-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Putative Holliday junction resolvase (Mkms_2394) CSB-YP378327MOM
CSB-EP378327MOM
CSB-BP378327MOM
CSB-MP378327MOM
CSB-EP378327MOM-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. N utilization substance protein B homolog (nusB) CSB-YP378328MOM
CSB-EP378328MOM
CSB-BP378328MOM
CSB-MP378328MOM
CSB-EP378328MOM-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達(dá)系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子，它攜帶特定的基因進(jìn)入宿主細(xì)胞，并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá)，經(jīng)過多年的發(fā)展，表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示，本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條：如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用，對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達(dá)服務(wù)，您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等，您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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