Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Burkholderia mallei ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP379710BPR
CSB-EP379710BPR
CSB-BP379710BPR
CSB-MP379710BPR
CSB-EP379710BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Flagellar hook-basal body complex protein FliE (fliE) CSB-YP379711BPR
CSB-EP379711BPR
CSB-BP379711BPR
CSB-MP379711BPR
CSB-EP379711BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei tRNA 2-thiocytidine biosynthesis protein TtcA (ttcA) CSB-YP379712BPR
CSB-EP379712BPR
CSB-BP379712BPR
CSB-MP379712BPR
CSB-EP379712BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Isocitrate dehydrogenase kinase/phosphatase (aceK), partial CSB-YP379713BPR
CSB-EP379713BPR
CSB-BP379713BPR
CSB-MP379713BPR
CSB-EP379713BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 30S ribosomal protein S4 (rpsD) CSB-YP379714BPR
CSB-EP379714BPR
CSB-BP379714BPR
CSB-MP379714BPR
CSB-EP379714BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 30S ribosomal protein S13 (rpsM) CSB-YP379715BPR
CSB-EP379715BPR
CSB-BP379715BPR
CSB-MP379715BPR
CSB-EP379715BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 50S ribosomal protein L15 (rplO) CSB-YP379716BPR
CSB-EP379716BPR
CSB-BP379716BPR
CSB-MP379716BPR
CSB-EP379716BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 50S ribosomal protein L5 (rplE) CSB-YP379717BPR
CSB-EP379717BPR
CSB-BP379717BPR
CSB-MP379717BPR
CSB-EP379717BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 50S ribosomal protein L14 (rplN) CSB-YP379718BPR
CSB-EP379718BPR
CSB-BP379718BPR
CSB-MP379718BPR
CSB-EP379718BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 30S ribosomal protein S3 (rpsC) CSB-YP379719BPR
CSB-EP379719BPR
CSB-BP379719BPR
CSB-MP379719BPR
CSB-EP379719BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 50S ribosomal protein L23 (rplW) CSB-YP379720BPR
CSB-EP379720BPR
CSB-BP379720BPR
CSB-MP379720BPR
CSB-EP379720BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 30S ribosomal protein S12 (rpsL) CSB-YP379721BPR
CSB-EP379721BPR
CSB-BP379721BPR
CSB-MP379721BPR
CSB-EP379721BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 50S ribosomal protein L11 (rplK) CSB-YP379722BPR
CSB-EP379722BPR
CSB-BP379722BPR
CSB-MP379722BPR
CSB-EP379722BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP379723BPR
CSB-EP379723BPR
CSB-BP379723BPR
CSB-MP379723BPR
CSB-EP379723BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Sec-independent protein translocase protein TatA (tatA), partial CSB-YP379724BPR
CSB-EP379724BPR
CSB-BP379724BPR
CSB-MP379724BPR
CSB-EP379724BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei ATP synthase epsilon chain (atpC) CSB-YP379725BPR
CSB-EP379725BPR
CSB-BP379725BPR
CSB-MP379725BPR
CSB-EP379725BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP379726BPR
CSB-EP379726BPR
CSB-BP379726BPR
CSB-MP379726BPR
CSB-EP379726BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Flagellar transcriptional regulator FlhD (flhD) CSB-YP379727BPR
CSB-EP379727BPR
CSB-BP379727BPR
CSB-MP379727BPR
CSB-EP379727BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris subsp. vulgaris Dihydroxy-acid dehydratase (ilvD), partial CSB-YP379728DHY
CSB-EP379728DHY
CSB-BP379728DHY
CSB-MP379728DHY
CSB-EP379728DHY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris subsp. vulgaris UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP379729DHY
CSB-EP379729DHY
CSB-BP379729DHY
CSB-MP379729DHY
CSB-EP379729DHY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>