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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bartonella bacilliformis Probable chorismate--pyruvate lyase (ubiC) CSB-YP379170BOR
CSB-EP379170BOR
CSB-BP379170BOR
CSB-MP379170BOR
CSB-EP379170BOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella bacilliformis Transcription elongation factor GreA (greA) CSB-YP379171BOR
CSB-EP379171BOR
CSB-BP379171BOR
CSB-MP379171BOR
CSB-EP379171BOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella bacilliformis tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP379172BOR
CSB-EP379172BOR
CSB-BP379172BOR
CSB-MP379172BOR
CSB-EP379172BOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella bacilliformis 50S ribosomal protein L34 (rpmH) CSB-YP379173BOR
CSB-EP379173BOR
CSB-BP379173BOR
CSB-MP379173BOR
CSB-EP379173BOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella bacilliformis 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP379174BOR
CSB-EP379174BOR
CSB-BP379174BOR
CSB-MP379174BOR
CSB-EP379174BOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella bacilliformis UPF0176 protein BARBAKC583_1091 (BARBAKC583_1091) CSB-YP379175BOR
CSB-EP379175BOR
CSB-BP379175BOR
CSB-MP379175BOR
CSB-EP379175BOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella bacilliformis Flagellar L-ring protein (flgH) CSB-YP379176BOR
CSB-EP379176BOR
CSB-BP379176BOR
CSB-MP379176BOR
CSB-EP379176BOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella bacilliformis Histidine--tRNA ligase (hisS) CSB-YP379177BOR
CSB-EP379177BOR
CSB-BP379177BOR
CSB-MP379177BOR
CSB-EP379177BOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella bacilliformis DNA mismatch repair protein mutL (mutL), partial CSB-YP379178BOR
CSB-EP379178BOR
CSB-BP379178BOR
CSB-MP379178BOR
CSB-EP379178BOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella bacilliformis Probable rRNA maturation factor (BARBAKC583_1245) CSB-YP379179BOR
CSB-EP379179BOR
CSB-BP379179BOR
CSB-MP379179BOR
CSB-EP379179BOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella bacilliformis Ribosome-binding factor A (rbfA) CSB-YP379180BOR
CSB-EP379180BOR
CSB-BP379180BOR
CSB-MP379180BOR
CSB-EP379180BOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella bacilliformis ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP379181BOR
CSB-EP379181BOR
CSB-BP379181BOR
CSB-MP379181BOR
CSB-EP379181BOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella bacilliformis 30S ribosomal protein S20 (rpsT) CSB-YP379182BOR
CSB-EP379182BOR
CSB-BP379182BOR
CSB-MP379182BOR
CSB-EP379182BOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella bacilliformis Chaperone protein DnaK (dnaK), partial CSB-YP379183BOR
CSB-EP379183BOR
CSB-BP379183BOR
CSB-MP379183BOR
CSB-EP379183BOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella bacilliformis Ubiquinone/menaquinone biosynthesis methyltransferase ubiE (ubiE) CSB-YP379184BOR
CSB-EP379184BOR
CSB-BP379184BOR
CSB-MP379184BOR
CSB-EP379184BOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella bacilliformis Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP379185BOR
CSB-EP379185BOR
CSB-BP379185BOR
CSB-MP379185BOR
CSB-EP379185BOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 1-aminocyclopropane-1-carboxylate deaminase (acdS) CSB-YP379186BPR
CSB-EP379186BPR
CSB-BP379186BPR
CSB-MP379186BPR
CSB-EP379186BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei NADH-quinone oxidoreductase subunit B 2 (nuoB2) CSB-YP379187BPR
CSB-EP379187BPR
CSB-BP379187BPR
CSB-MP379187BPR
CSB-EP379187BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei ATP synthase subunit alpha 2 (atpA2), partial CSB-YP379188BPR
CSB-EP379188BPR
CSB-BP379188BPR
CSB-MP379188BPR
CSB-EP379188BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 50S ribosomal protein L25 (rplY) CSB-YP379189BPR
CSB-EP379189BPR
CSB-BP379189BPR
CSB-MP379189BPR
CSB-EP379189BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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