Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Polaromonas naphthalenivorans UPF0434 protein Pnap_1922 (Pnap_1922) CSB-YP379370PYT
CSB-EP379370PYT
CSB-BP379370PYT
CSB-MP379370PYT
CSB-EP379370PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans Ribulose bisphosphate carboxylase (cbbM) CSB-YP379371PYT
CSB-EP379371PYT
CSB-BP379371PYT
CSB-MP379371PYT
CSB-EP379371PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans Protein TolB (tolB) CSB-YP379372PYT
CSB-EP379372PYT
CSB-BP379372PYT
CSB-MP379372PYT
CSB-EP379372PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans Nitrogenase-stabilizing/protective protein nifW (nifW) CSB-YP379373PYT
CSB-EP379373PYT
CSB-BP379373PYT
CSB-MP379373PYT
CSB-EP379373PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans ATP synthase subunit beta 2 (atpD2) CSB-YP379374PYT
CSB-EP379374PYT
CSB-BP379374PYT
CSB-MP379374PYT
CSB-EP379374PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans Alkanesulfonate monooxygenase (ssuD) CSB-YP379375PYT
CSB-EP379375PYT
CSB-BP379375PYT
CSB-MP379375PYT
CSB-EP379375PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP379376PYT
CSB-EP379376PYT
CSB-BP379376PYT
CSB-MP379376PYT
CSB-EP379376PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans Chorismate synthase (aroC) CSB-YP379377PYT
CSB-EP379377PYT
CSB-BP379377PYT
CSB-MP379377PYT
CSB-EP379377PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans 50S ribosomal protein L35 (rpmI) CSB-YP379378PYT
CSB-EP379378PYT
CSB-BP379378PYT
CSB-MP379378PYT
CSB-EP379378PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP379379PYT1
CSB-EP379379PYT1
CSB-BP379379PYT1
CSB-MP379379PYT1
CSB-EP379379PYT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans Cysteine--tRNA ligase (cysS) CSB-YP379380PYT
CSB-EP379380PYT
CSB-BP379380PYT
CSB-MP379380PYT
CSB-EP379380PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans Glutamyl-Q tRNA (Asp) synthetase CSB-YP379381PYT
CSB-EP379381PYT
CSB-BP379381PYT
CSB-MP379381PYT
CSB-EP379381PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans 30S ribosomal protein S20 (rpsT) CSB-YP379382PYT
CSB-EP379382PYT
CSB-BP379382PYT
CSB-MP379382PYT
CSB-EP379382PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP379383PYT
CSB-EP379383PYT
CSB-BP379383PYT
CSB-MP379383PYT
CSB-EP379383PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans Elongation factor 4 (lepA), partial CSB-YP379384PYT
CSB-EP379384PYT
CSB-BP379384PYT
CSB-MP379384PYT
CSB-EP379384PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans 50S ribosomal protein L32 (rpmF) CSB-YP379385PYT
CSB-EP379385PYT
CSB-BP379385PYT
CSB-MP379385PYT
CSB-EP379385PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans 50S ribosomal protein L33 (rpmG) CSB-YP379386PYT
CSB-EP379386PYT
CSB-BP379386PYT
CSB-MP379386PYT
CSB-EP379386PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP379387PYT
CSB-EP379387PYT
CSB-BP379387PYT
CSB-MP379387PYT
CSB-EP379387PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (lpxC) CSB-YP379388PYT
CSB-EP379388PYT
CSB-BP379388PYT
CSB-MP379388PYT
CSB-EP379388PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP379389PYT
CSB-EP379389PYT
CSB-BP379389PYT
CSB-MP379389PYT
CSB-EP379389PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>