Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Polaromonas naphthalenivorans 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP379350PYT
CSB-EP379350PYT
CSB-BP379350PYT
CSB-MP379350PYT
CSB-EP379350PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans Pantothenate synthetase (panC) CSB-YP379351PYT
CSB-EP379351PYT
CSB-BP379351PYT
CSB-MP379351PYT
CSB-EP379351PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans Urease subunit alpha (ureC), partial CSB-YP379352PYT
CSB-EP379352PYT
CSB-BP379352PYT
CSB-MP379352PYT
CSB-EP379352PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans Translation initiation factor IF-1 (infA) CSB-YP379353PYT
CSB-EP379353PYT
CSB-BP379353PYT
CSB-MP379353PYT
CSB-EP379353PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans Fructose-1,6-bisphosphatase class 1 1 (fbp1) CSB-YP379354PYT
CSB-EP379354PYT
CSB-BP379354PYT
CSB-MP379354PYT
CSB-EP379354PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans UPF0234 protein Pnap_1080 (Pnap_1080) CSB-YP379355PYT
CSB-EP379355PYT
CSB-BP379355PYT
CSB-MP379355PYT
CSB-EP379355PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans Glycogen synthase (glgA) CSB-YP379356PYT
CSB-EP379356PYT
CSB-BP379356PYT
CSB-MP379356PYT
CSB-EP379356PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans Enolase (eno) CSB-YP379357PYT
CSB-EP379357PYT
CSB-BP379357PYT
CSB-MP379357PYT
CSB-EP379357PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans Undecaprenyl-diphosphatase (uppP), partial CSB-YP379358PYT1
CSB-EP379358PYT1
CSB-BP379358PYT1
CSB-MP379358PYT1
CSB-EP379358PYT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP379359PYT
CSB-EP379359PYT
CSB-BP379359PYT
CSB-MP379359PYT
CSB-EP379359PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans NADH-quinone oxidoreductase subunit D (nuoD) CSB-YP379360PYT
CSB-EP379360PYT
CSB-BP379360PYT
CSB-MP379360PYT
CSB-EP379360PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans GMP reductase (guaC) CSB-YP379361PYT
CSB-EP379361PYT
CSB-BP379361PYT
CSB-MP379361PYT
CSB-EP379361PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP379362PYT
CSB-EP379362PYT
CSB-BP379362PYT
CSB-MP379362PYT
CSB-EP379362PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans Chaperone protein DnaJ (dnaJ) CSB-YP379363PYT
CSB-EP379363PYT
CSB-BP379363PYT
CSB-MP379363PYT
CSB-EP379363PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans Putative iron-sulfur cluster insertion protein ErpA 2 (erpA2) CSB-YP379364PYT
CSB-EP379364PYT
CSB-BP379364PYT
CSB-MP379364PYT
CSB-EP379364PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans Ribonuclease H (rnhA) CSB-YP379365PYT
CSB-EP379365PYT
CSB-BP379365PYT
CSB-MP379365PYT
CSB-EP379365PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans Coproporphyrinogen-III oxidase, aerobic (hemF) CSB-YP379366PYT
CSB-EP379366PYT
CSB-BP379366PYT
CSB-MP379366PYT
CSB-EP379366PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP379367PYT
CSB-EP379367PYT
CSB-BP379367PYT
CSB-MP379367PYT
CSB-EP379367PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans Probable intracellular septation protein A (Pnap_1834), partial CSB-YP379368PYT1
CSB-EP379368PYT1
CSB-BP379368PYT1
CSB-MP379368PYT1
CSB-EP379368PYT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans GTPase Der (der) CSB-YP379369PYT
CSB-EP379369PYT
CSB-BP379369PYT
CSB-MP379369PYT
CSB-EP379369PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>