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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bartonella bacilliformis Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP380070BOR
CSB-EP380070BOR
CSB-BP380070BOR
CSB-MP380070BOR
CSB-EP380070BOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella bacilliformis (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP380071BOR
CSB-EP380071BOR
CSB-BP380071BOR
CSB-MP380071BOR
CSB-EP380071BOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella bacilliformis 30S ribosomal protein S15 (rpsO) CSB-YP380072BOR
CSB-EP380072BOR
CSB-BP380072BOR
CSB-MP380072BOR
CSB-EP380072BOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella bacilliformis Pantothenate kinase (coaA) CSB-YP380073BOR
CSB-EP380073BOR
CSB-BP380073BOR
CSB-MP380073BOR
CSB-EP380073BOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella bacilliformis Cytidylate kinase (cmk) CSB-YP380074BOR
CSB-EP380074BOR
CSB-BP380074BOR
CSB-MP380074BOR
CSB-EP380074BOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella bacilliformis Acetylglutamate kinase (argB) CSB-YP380075BOR
CSB-EP380075BOR
CSB-BP380075BOR
CSB-MP380075BOR
CSB-EP380075BOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella bacilliformis Succinyl-diaminopimelate desuccinylase (dapE) CSB-YP380076BOR
CSB-EP380076BOR
CSB-BP380076BOR
CSB-MP380076BOR
CSB-EP380076BOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Dictomallein (dtmL), partial CSB-YP380077BPR
CSB-EP380077BPR
CSB-BP380077BPR
CSB-MP380077BPR
CSB-EP380077BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei NH (3)-dependent NAD (+) synthetase CSB-YP380078BPR
CSB-EP380078BPR
CSB-BP380078BPR
CSB-MP380078BPR
CSB-EP380078BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Glutamyl-tRNA reductase (hemA) CSB-YP380079BPR
CSB-EP380079BPR
CSB-BP380079BPR
CSB-MP380079BPR
CSB-EP380079BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP380080BPR
CSB-EP380080BPR
CSB-BP380080BPR
CSB-MP380080BPR
CSB-EP380080BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei UPF0102 protein BMASAVP1_A0024 (BMASAVP1_A0024) CSB-YP380081BPR
CSB-EP380081BPR
CSB-BP380081BPR
CSB-MP380081BPR
CSB-EP380081BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP380082BPR
CSB-EP380082BPR
CSB-BP380082BPR
CSB-MP380082BPR
CSB-EP380082BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Peptidyl-tRNA hydrolase (pth) CSB-YP380083BPR
CSB-EP380083BPR
CSB-BP380083BPR
CSB-MP380083BPR
CSB-EP380083BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Type III pantothenate kinase (coaX) CSB-YP380084BPR
CSB-EP380084BPR
CSB-BP380084BPR
CSB-MP380084BPR
CSB-EP380084BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP380085BPR
CSB-EP380085BPR
CSB-BP380085BPR
CSB-MP380085BPR
CSB-EP380085BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP380086BPR
CSB-EP380086BPR
CSB-BP380086BPR
CSB-MP380086BPR
CSB-EP380086BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Ribosomal protein L11 methyltransferase (prmA) CSB-YP380087BPR
CSB-EP380087BPR
CSB-BP380087BPR
CSB-MP380087BPR
CSB-EP380087BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei RNA pyrophosphohydrolase (rppH) CSB-YP380088BPR
CSB-EP380088BPR
CSB-BP380088BPR
CSB-MP380088BPR
CSB-EP380088BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei UPF0289 protein BMASAVP1_A0457 (BMASAVP1_A0457) CSB-YP380089BPR
CSB-EP380089BPR
CSB-BP380089BPR
CSB-MP380089BPR
CSB-EP380089BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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