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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Verminephrobacter eiseniae 50S ribosomal protein L7/L12 (rplL) CSB-YP380030VDX
CSB-EP380030VDX
CSB-BP380030VDX
CSB-MP380030VDX
CSB-EP380030VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae 30S ribosomal protein S11 (rpsK) CSB-YP380031VDX
CSB-EP380031VDX
CSB-BP380031VDX
CSB-MP380031VDX
CSB-EP380031VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae 50S ribosomal protein L24 (rplX) CSB-YP380032VDX
CSB-EP380032VDX
CSB-BP380032VDX
CSB-MP380032VDX
CSB-EP380032VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae RNA pyrophosphohydrolase (rppH) CSB-YP380033VDX
CSB-EP380033VDX
CSB-BP380033VDX
CSB-MP380033VDX
CSB-EP380033VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae 10 kDa chaperonin (groS) CSB-YP380034VDX
CSB-EP380034VDX
CSB-BP380034VDX
CSB-MP380034VDX
CSB-EP380034VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae 30S ribosomal protein S9 (rpsI) CSB-YP380035VDX
CSB-EP380035VDX
CSB-BP380035VDX
CSB-MP380035VDX
CSB-EP380035VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae NADH-quinone oxidoreductase subunit H (nuoH), partial CSB-YP380036VDX
CSB-EP380036VDX
CSB-BP380036VDX
CSB-MP380036VDX
CSB-EP380036VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae NADH-quinone oxidoreductase subunit A (nuoA), partial CSB-YP380037VDX1
CSB-EP380037VDX1
CSB-BP380037VDX1
CSB-MP380037VDX1
CSB-EP380037VDX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP380038VDX
CSB-EP380038VDX
CSB-BP380038VDX
CSB-MP380038VDX
CSB-EP380038VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP380039VDX
CSB-EP380039VDX
CSB-BP380039VDX
CSB-MP380039VDX
CSB-EP380039VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae Adenylosuccinate synthetase (purA) CSB-YP380040VDX
CSB-EP380040VDX
CSB-BP380040VDX
CSB-MP380040VDX
CSB-EP380040VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae Elongation factor 4 (lepA), partial CSB-YP380041VDX
CSB-EP380041VDX
CSB-BP380041VDX
CSB-MP380041VDX
CSB-EP380041VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae 50S ribosomal protein L32 (rpmF) CSB-YP380042VDX
CSB-EP380042VDX
CSB-BP380042VDX
CSB-MP380042VDX
CSB-EP380042VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae 2-isopropylmalate synthase (leuA), partial CSB-YP380043VDX
CSB-EP380043VDX
CSB-BP380043VDX
CSB-MP380043VDX
CSB-EP380043VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae Glutamate 5-kinase (proB) CSB-YP380044VDX
CSB-EP380044VDX
CSB-BP380044VDX
CSB-MP380044VDX
CSB-EP380044VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae 30S ribosomal protein S16 (rpsP) CSB-YP380045VDX
CSB-EP380045VDX
CSB-BP380045VDX
CSB-MP380045VDX
CSB-EP380045VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP380046VDX
CSB-EP380046VDX
CSB-BP380046VDX
CSB-MP380046VDX
CSB-EP380046VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP380047VDX
CSB-EP380047VDX
CSB-BP380047VDX
CSB-MP380047VDX
CSB-EP380047VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae Probable manganese transport protein MntH (mntH), partial CSB-YP380048VDX
CSB-EP380048VDX
CSB-BP380048VDX
CSB-MP380048VDX
CSB-EP380048VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Verminephrobacter eiseniae Methionine--tRNA ligase (metG), partial CSB-YP380049VDX
CSB-EP380049VDX
CSB-BP380049VDX
CSB-MP380049VDX
CSB-EP380049VDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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