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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Polaromonas naphthalenivorans 4-hydroxy-2-oxovalerate aldolase (Pnap_4142) CSB-YP380810PYT
CSB-EP380810PYT
CSB-BP380810PYT
CSB-MP380810PYT
CSB-EP380810PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans Acetaldehyde dehydrogenase (Pnap_4143) CSB-YP380811PYT
CSB-EP380811PYT
CSB-BP380811PYT
CSB-MP380811PYT
CSB-EP380811PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. jejuni serotype O:23/36 Ribonuclease HII (rnhB) CSB-YP380812CWF
CSB-EP380812CWF
CSB-BP380812CWF
CSB-MP380812CWF
CSB-EP380812CWF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. jejuni serotype O:23/36 tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP380813CWF
CSB-EP380813CWF
CSB-BP380813CWF
CSB-MP380813CWF
CSB-EP380813CWF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. jejuni serotype O:23/36 GTPase obg (obg) CSB-YP380814CWF
CSB-EP380814CWF
CSB-BP380814CWF
CSB-MP380814CWF
CSB-EP380814CWF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. jejuni serotype O:23/36 ATP synthase gamma chain (atpG) CSB-YP380815CWF
CSB-EP380815CWF
CSB-BP380815CWF
CSB-MP380815CWF
CSB-EP380815CWF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. jejuni serotype O:23/36 Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP380816CWF
CSB-EP380816CWF
CSB-BP380816CWF
CSB-MP380816CWF
CSB-EP380816CWF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. jejuni serotype O:23/36 UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (lpxC) CSB-YP380817CWF
CSB-EP380817CWF
CSB-BP380817CWF
CSB-MP380817CWF
CSB-EP380817CWF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. jejuni serotype O:23/36 UPF0102 protein CJJ81176_0184 (CJJ81176_0184) CSB-YP380818CWF
CSB-EP380818CWF
CSB-BP380818CWF
CSB-MP380818CWF
CSB-EP380818CWF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. jejuni serotype O:23/36 Dihydrodipicolinate reductase (dapB) CSB-YP380819CWF
CSB-EP380819CWF
CSB-BP380819CWF
CSB-MP380819CWF
CSB-EP380819CWF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. jejuni serotype O:23/36 N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP380820CWF
CSB-EP380820CWF
CSB-BP380820CWF
CSB-MP380820CWF
CSB-EP380820CWF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. jejuni serotype O:23/36 50S ribosomal protein L20 (rplT) CSB-YP380821CWF
CSB-EP380821CWF
CSB-BP380821CWF
CSB-MP380821CWF
CSB-EP380821CWF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. jejuni serotype O:23/36 50S ribosomal protein L25 (rplY) CSB-YP380822CWF
CSB-EP380822CWF
CSB-BP380822CWF
CSB-MP380822CWF
CSB-EP380822CWF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. jejuni serotype O:23/36 Exodeoxyribonuclease 7 large subunit (xseA) CSB-YP380823CWF
CSB-EP380823CWF
CSB-BP380823CWF
CSB-MP380823CWF
CSB-EP380823CWF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. jejuni serotype O:23/36 50S ribosomal protein L32 (rpmF) CSB-YP380824CWF
CSB-EP380824CWF
CSB-BP380824CWF
CSB-MP380824CWF
CSB-EP380824CWF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. jejuni serotype O:23/36 Tryptophan synthase alpha chain (trpA) CSB-YP380825CWF
CSB-EP380825CWF
CSB-BP380825CWF
CSB-MP380825CWF
CSB-EP380825CWF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. jejuni serotype O:23/36 Phosphoglucosamine mutase (glmM) CSB-YP380826CWF
CSB-EP380826CWF
CSB-BP380826CWF
CSB-MP380826CWF
CSB-EP380826CWF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. jejuni serotype O:23/36 2-dehydro-3-deoxyphosphooctonate aldolase (kdsA) CSB-YP380827CWF
CSB-EP380827CWF
CSB-BP380827CWF
CSB-MP380827CWF
CSB-EP380827CWF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. jejuni serotype O:23/36 Lysine--tRNA ligase (lysS), partial CSB-YP380828CWF
CSB-EP380828CWF
CSB-BP380828CWF
CSB-MP380828CWF
CSB-EP380828CWF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. jejuni serotype O:23/36 50S ribosomal protein L28 (rpmB) CSB-YP380829CWF
CSB-EP380829CWF
CSB-BP380829CWF
CSB-MP380829CWF
CSB-EP380829CWF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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