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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Polaromonas naphthalenivorans Probable Fe (2+)-trafficking protein CSB-YP380790PYT
CSB-EP380790PYT
CSB-BP380790PYT
CSB-MP380790PYT
CSB-EP380790PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans 23S rRNA (uracil (1939)-C (5))-methyltransferase RlmD CSB-YP380791PYT
CSB-EP380791PYT
CSB-BP380791PYT
CSB-MP380791PYT
CSB-EP380791PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans Phosphoglucosamine mutase (glmM) CSB-YP380792PYT
CSB-EP380792PYT
CSB-BP380792PYT
CSB-MP380792PYT
CSB-EP380792PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans Dihydroorotate dehydrogenase (quinone) CSB-YP380793PYT
CSB-EP380793PYT
CSB-BP380793PYT
CSB-MP380793PYT
CSB-EP380793PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans Threonine--tRNA ligase (thrS), partial CSB-YP380794PYT
CSB-EP380794PYT
CSB-BP380794PYT
CSB-MP380794PYT
CSB-EP380794PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans Trigger factor (tig) CSB-YP380795PYT
CSB-EP380795PYT
CSB-BP380795PYT
CSB-MP380795PYT
CSB-EP380795PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans Tryptophan synthase beta chain (trpB) CSB-YP380796PYT
CSB-EP380796PYT
CSB-BP380796PYT
CSB-MP380796PYT
CSB-EP380796PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans DNA repair protein recO (recO) CSB-YP380797PYT
CSB-EP380797PYT
CSB-BP380797PYT
CSB-MP380797PYT
CSB-EP380797PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans 50S ribosomal protein L28 (rpmB) CSB-YP380798PYT
CSB-EP380798PYT
CSB-BP380798PYT
CSB-MP380798PYT
CSB-EP380798PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans UPF0502 protein Pnap_3223 (Pnap_3223) CSB-YP380799PYT
CSB-EP380799PYT
CSB-BP380799PYT
CSB-MP380799PYT
CSB-EP380799PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP380800PYT
CSB-EP380800PYT
CSB-BP380800PYT
CSB-MP380800PYT
CSB-EP380800PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP380801PYT
CSB-EP380801PYT
CSB-BP380801PYT
CSB-MP380801PYT
CSB-EP380801PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP380802PYT
CSB-EP380802PYT
CSB-BP380802PYT
CSB-MP380802PYT
CSB-EP380802PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans Acetylglutamate kinase (argB) CSB-YP380803PYT
CSB-EP380803PYT
CSB-BP380803PYT
CSB-MP380803PYT
CSB-EP380803PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans Chaperone protein htpG (htpG), partial CSB-YP380804PYT
CSB-EP380804PYT
CSB-BP380804PYT
CSB-MP380804PYT
CSB-EP380804PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans Potassium-transporting ATPase A chain (kdpA), partial CSB-YP380805PYT
CSB-EP380805PYT
CSB-BP380805PYT
CSB-MP380805PYT
CSB-EP380805PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans 50S ribosomal protein L1 (rplA) CSB-YP380806PYT
CSB-EP380806PYT
CSB-BP380806PYT
CSB-MP380806PYT
CSB-EP380806PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans Arginine--tRNA ligase (argS), partial CSB-YP380807PYT
CSB-EP380807PYT
CSB-BP380807PYT
CSB-MP380807PYT
CSB-EP380807PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans HPr kinase/phosphorylase (hprK) CSB-YP380808PYT
CSB-EP380808PYT
CSB-BP380808PYT
CSB-MP380808PYT
CSB-EP380808PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans D- (-)-3-hydroxybutyrate oligomer hydrolase, partial CSB-YP380809PYT
CSB-EP380809PYT
CSB-BP380809PYT
CSB-MP380809PYT
CSB-EP380809PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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