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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Halorhodospira halophila Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP381130HSG
CSB-EP381130HSG
CSB-BP381130HSG
CSB-MP381130HSG
CSB-EP381130HSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halorhodospira halophila Gamma-glutamyl phosphate reductase (proA) CSB-YP381131HSG
CSB-EP381131HSG
CSB-BP381131HSG
CSB-MP381131HSG
CSB-EP381131HSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halorhodospira halophila Probable chemoreceptor glutamine deamidase CheD (cheD) CSB-YP381132HSG
CSB-EP381132HSG
CSB-BP381132HSG
CSB-MP381132HSG
CSB-EP381132HSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halorhodospira halophila Probable transcriptional regulatory protein Hhal_2210 (Hhal_2210) CSB-YP381133HSG
CSB-EP381133HSG
CSB-BP381133HSG
CSB-MP381133HSG
CSB-EP381133HSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halorhodospira halophila Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP381134HSG
CSB-EP381134HSG
CSB-BP381134HSG
CSB-MP381134HSG
CSB-EP381134HSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halorhodospira halophila UPF0278 protein Hhal_2243 (Hhal_2243) CSB-YP381135HSG
CSB-EP381135HSG
CSB-BP381135HSG
CSB-MP381135HSG
CSB-EP381135HSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halorhodospira halophila 50S ribosomal protein L31 type B (rpmE2) CSB-YP381136HSG
CSB-EP381136HSG
CSB-BP381136HSG
CSB-MP381136HSG
CSB-EP381136HSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halorhodospira halophila Shikimate kinase (aroK) CSB-YP381137HSG
CSB-EP381137HSG
CSB-BP381137HSG
CSB-MP381137HSG
CSB-EP381137HSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halorhodospira halophila 3-octaprenyl-4-hydroxybenzoate carboxy-lyase (ubiD) CSB-YP381138HSG
CSB-EP381138HSG
CSB-BP381138HSG
CSB-MP381138HSG
CSB-EP381138HSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halorhodospira halophila Acetylglutamate kinase (argB) CSB-YP381139HSG
CSB-EP381139HSG
CSB-BP381139HSG
CSB-MP381139HSG
CSB-EP381139HSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halorhodospira halophila UPF0758 protein Hhal_2301 (Hhal_2301) CSB-YP381140HSG
CSB-EP381140HSG
CSB-BP381140HSG
CSB-MP381140HSG
CSB-EP381140HSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halorhodospira halophila Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP381141HSG
CSB-EP381141HSG
CSB-BP381141HSG
CSB-MP381141HSG
CSB-EP381141HSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halorhodospira halophila 50S ribosomal protein L33 (rpmG) CSB-YP381142HSG
CSB-EP381142HSG
CSB-BP381142HSG
CSB-MP381142HSG
CSB-EP381142HSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halorhodospira halophila Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP381143HSG
CSB-EP381143HSG
CSB-BP381143HSG
CSB-MP381143HSG
CSB-EP381143HSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halorhodospira halophila Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP381144HSG1
CSB-EP381144HSG1
CSB-BP381144HSG1
CSB-MP381144HSG1
CSB-EP381144HSG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halorhodospira halophila 3-dehydroquinate dehydratase (aroQ) CSB-YP381145HSG
CSB-EP381145HSG
CSB-BP381145HSG
CSB-MP381145HSG
CSB-EP381145HSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halorhodospira halophila 10 kDa chaperonin (groS) CSB-YP381146HSG
CSB-EP381146HSG
CSB-BP381146HSG
CSB-MP381146HSG
CSB-EP381146HSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halorhodospira halophila Sulfate adenylyltransferase subunit 2 (cysD) CSB-YP381147HSG
CSB-EP381147HSG
CSB-BP381147HSG
CSB-MP381147HSG
CSB-EP381147HSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halorhodospira halophila ATP synthase epsilon chain (atpC) CSB-YP381148HSG
CSB-EP381148HSG
CSB-BP381148HSG
CSB-MP381148HSG
CSB-EP381148HSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Perna viridis Foot protein 1 variant 1 (fp-1), partial CSB-YP381149PYH
CSB-EP381149PYH
CSB-BP381149PYH
CSB-MP381149PYH
CSB-EP381149PYH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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