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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Aspergillus niger Vacuolar protein sorting/targeting protein 10 (vps10), partial CSB-YP381533AVE
CSB-EP381533AVE
CSB-BP381533AVE
CSB-MP381533AVE
CSB-EP381533AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Ribosome biogenesis protein ytm1 (ytm1) CSB-YP381534AVE
CSB-EP381534AVE
CSB-BP381534AVE
CSB-MP381534AVE
CSB-EP381534AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Elongation factor G, mitochondrial (mef1), partial CSB-YP381535AVE
CSB-EP381535AVE
CSB-BP381535AVE
CSB-MP381535AVE
CSB-EP381535AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Pre-mRNA-splicing ATP-dependent RNA helicase prp28 (prp28), partial CSB-YP381536AVE
CSB-EP381536AVE
CSB-BP381536AVE
CSB-MP381536AVE
CSB-EP381536AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Plasma membrane fusion protein prm1 (prm1), partial CSB-YP381537AVE1
CSB-EP381537AVE1
CSB-BP381537AVE1
CSB-MP381537AVE1
CSB-EP381537AVE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Mitochondrial distribution and morphology protein 34 (mdm34), partial CSB-YP381538AVE
CSB-EP381538AVE
CSB-BP381538AVE
CSB-MP381538AVE
CSB-EP381538AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Probable pectinesterase A (pmeA) CSB-YP381539AVE
CSB-EP381539AVE
CSB-BP381539AVE
CSB-MP381539AVE
CSB-EP381539AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Leukotriene A-4 hydrolase homolog (An05g00070), partial CSB-YP381540AVE
CSB-EP381540AVE
CSB-BP381540AVE
CSB-MP381540AVE
CSB-EP381540AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Mitochondrial inner membrane protease atp23 (atp23) CSB-YP381541AVE
CSB-EP381541AVE
CSB-BP381541AVE
CSB-MP381541AVE
CSB-EP381541AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Probable alpha-galactosidase A (aglA), partial CSB-YP381542AVE
CSB-EP381542AVE
CSB-BP381542AVE
CSB-MP381542AVE
CSB-EP381542AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger 1,3-beta-glucan synthase component FKS1 (fksA), partial CSB-YP381543AVE
CSB-EP381543AVE
CSB-BP381543AVE
CSB-MP381543AVE
CSB-EP381543AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Probable rhamnogalacturonase C (rhgC) CSB-YP381544AVE
CSB-EP381544AVE
CSB-BP381544AVE
CSB-MP381544AVE
CSB-EP381544AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Protein rot1 (rot1), partial CSB-YP381545AVE
CSB-EP381545AVE
CSB-BP381545AVE
CSB-MP381545AVE
CSB-EP381545AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Sterol 3-beta-glucosyltransferase (atg26), partial CSB-YP381546AVE
CSB-EP381546AVE
CSB-BP381546AVE
CSB-MP381546AVE
CSB-EP381546AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger rRNA-processing protein efg1 (efg1) CSB-YP381547AVE
CSB-EP381547AVE
CSB-BP381547AVE
CSB-MP381547AVE
CSB-EP381547AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Kynurenine 3-monooxygenase 3 (bna4-3) CSB-YP381548AVE
CSB-EP381548AVE
CSB-BP381548AVE
CSB-MP381548AVE
CSB-EP381548AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Probable beta-glucosidase D (bglD), partial CSB-YP381549AVE
CSB-EP381549AVE
CSB-BP381549AVE
CSB-MP381549AVE
CSB-EP381549AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Histone H2A.Z-specific chaperone chz1 (chz1) CSB-YP381550AVE
CSB-EP381550AVE
CSB-BP381550AVE
CSB-MP381550AVE
CSB-EP381550AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Probable cytosolic iron-sulfur protein assembly protein 1 (cia1) CSB-YP381551AVE
CSB-EP381551AVE
CSB-BP381551AVE
CSB-MP381551AVE
CSB-EP381551AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Probable alpha-N-arabinofuranosidase C (abfC), partial CSB-YP381552AVE
CSB-EP381552AVE
CSB-BP381552AVE
CSB-MP381552AVE
CSB-EP381552AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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