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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Aspergillus niger Mitochondrial outer membrane protein iml2 (iml2), partial CSB-YP381553AVE1
CSB-EP381553AVE1
CSB-BP381553AVE1
CSB-MP381553AVE1
CSB-EP381553AVE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Probable quinate permease (qutD), partial CSB-YP381554AVE
CSB-EP381554AVE
CSB-BP381554AVE
CSB-MP381554AVE
CSB-EP381554AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Mitogen-activated protein kinase hog1 (hog1) CSB-YP381555AVE
CSB-EP381555AVE
CSB-BP381555AVE
CSB-MP381555AVE
CSB-EP381555AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger ATP-dependent RNA helicase dbp10 (dbp10), partial CSB-YP381556AVE
CSB-EP381556AVE
CSB-BP381556AVE
CSB-MP381556AVE
CSB-EP381556AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger ATP-dependent RNA helicase dbp4 (dbp4), partial CSB-YP381557AVE
CSB-EP381557AVE
CSB-BP381557AVE
CSB-MP381557AVE
CSB-EP381557AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Autophagy-related protein 2 (atg2), partial CSB-YP381558AVE
CSB-EP381558AVE
CSB-BP381558AVE
CSB-MP381558AVE
CSB-EP381558AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Probable arabinan endo-1,5-alpha-L-arabinosidase A (abnA) CSB-YP381559AVE
CSB-EP381559AVE
CSB-BP381559AVE
CSB-MP381559AVE
CSB-EP381559AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Translation factor guf1, mitochondrial (guf1), partial CSB-YP381560AVE
CSB-EP381560AVE
CSB-BP381560AVE
CSB-MP381560AVE
CSB-EP381560AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Probable pectate lyase A (plyA) CSB-YP381561AVE
CSB-EP381561AVE
CSB-BP381561AVE
CSB-MP381561AVE
CSB-EP381561AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Probable catabolite repression protein creC (creC), partial CSB-YP381562AVE
CSB-EP381562AVE
CSB-BP381562AVE
CSB-MP381562AVE
CSB-EP381562AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Class E vacuolar protein-sorting machinery protein hse1 (hse1), partial CSB-YP381563AVE
CSB-EP381563AVE
CSB-BP381563AVE
CSB-MP381563AVE
CSB-EP381563AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Probable beta-mannosidase A (mndA), partial CSB-YP381564AVE
CSB-EP381564AVE
CSB-BP381564AVE
CSB-MP381564AVE
CSB-EP381564AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger ATP-dependent RNA helicase chl1 (chl1), partial CSB-YP381565AVE
CSB-EP381565AVE
CSB-BP381565AVE
CSB-MP381565AVE
CSB-EP381565AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Histone H2B (htb1) CSB-YP381566AVE
CSB-EP381566AVE
CSB-BP381566AVE
CSB-MP381566AVE
CSB-EP381566AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Probable D-xylulose reductase A (xdhA) CSB-YP381567AVE
CSB-EP381567AVE
CSB-BP381567AVE
CSB-MP381567AVE
CSB-EP381567AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Probable 1,4-beta-D-glucan cellobiohydrolase C (cbhC) CSB-YP381568AVE
CSB-EP381568AVE
CSB-BP381568AVE
CSB-MP381568AVE
CSB-EP381568AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger ATP-dependent RNA helicase mrh4, mitochondrial (mrh4), partial CSB-YP381569AVE
CSB-EP381569AVE
CSB-BP381569AVE
CSB-MP381569AVE
CSB-EP381569AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Probable acetylxylan esterase A (axeA) CSB-YP381570AVE
CSB-EP381570AVE
CSB-BP381570AVE
CSB-MP381570AVE
CSB-EP381570AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Nascent polypeptide-associated complex subunit beta (egd1) CSB-YP381571AVE
CSB-EP381571AVE
CSB-BP381571AVE
CSB-MP381571AVE
CSB-EP381571AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Probable feruloyl esterase B (faeB), partial CSB-YP381572AVE
CSB-EP381572AVE
CSB-BP381572AVE
CSB-MP381572AVE
CSB-EP381572AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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