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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Prochlorococcus marinus Putative pterin-4-alpha-carbinolamine dehydratase (P9515_05551) CSB-YP381913PZG
CSB-EP381913PZG
CSB-BP381913PZG
CSB-MP381913PZG
CSB-EP381913PZG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP381914PZG
CSB-EP381914PZG
CSB-BP381914PZG
CSB-MP381914PZG
CSB-EP381914PZG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Light-independent protochlorophyllide reductase subunit N (chlN) CSB-YP381915PZG
CSB-EP381915PZG
CSB-BP381915PZG
CSB-MP381915PZG
CSB-EP381915PZG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Chromosomal replication initiator protein DnaA (dnaA) CSB-YP381916PZG
CSB-EP381916PZG
CSB-BP381916PZG
CSB-MP381916PZG
CSB-EP381916PZG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Homoserine kinase (thrB) CSB-YP381917PZG
CSB-EP381917PZG
CSB-BP381917PZG
CSB-MP381917PZG
CSB-EP381917PZG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Bifunctional protein GlmU (glmU) CSB-YP381918PZG
CSB-EP381918PZG
CSB-BP381918PZG
CSB-MP381918PZG
CSB-EP381918PZG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus D-fructose 1,6-bisphosphatase class 2/sedoheptulose 1,7-bisphosphatase (P9515_08161) CSB-YP381919PZG
CSB-EP381919PZG
CSB-BP381919PZG
CSB-MP381919PZG
CSB-EP381919PZG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Phosphopantetheine adenylyltransferase (coaD) CSB-YP381920PZG
CSB-EP381920PZG
CSB-BP381920PZG
CSB-MP381920PZG
CSB-EP381920PZG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Photosystem II reaction center Psb28 protein (psb28) CSB-YP381921PZG
CSB-EP381921PZG
CSB-BP381921PZG
CSB-MP381921PZG
CSB-EP381921PZG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP381922PZG
CSB-EP381922PZG
CSB-BP381922PZG
CSB-MP381922PZG
CSB-EP381922PZG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 30S ribosomal protein S21 (rpsU) CSB-YP381923PZG
CSB-EP381923PZG
CSB-BP381923PZG
CSB-MP381923PZG
CSB-EP381923PZG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus UPF0176 protein P9515_11851 (P9515_11851) CSB-YP381924PZG
CSB-EP381924PZG
CSB-BP381924PZG
CSB-MP381924PZG
CSB-EP381924PZG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Methylenetetrahydrofolate--tRNA- (uracil-5-)-methyltransferase TrmFO CSB-YP381925PZG
CSB-EP381925PZG
CSB-BP381925PZG
CSB-MP381925PZG
CSB-EP381925PZG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Photosystem I assembly protein Ycf4 (ycf4), partial CSB-YP381926PZG1
CSB-EP381926PZG1
CSB-BP381926PZG1
CSB-MP381926PZG1
CSB-EP381926PZG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Glycine--tRNA ligase alpha subunit (glyQ) CSB-YP381927PZG
CSB-EP381927PZG
CSB-BP381927PZG
CSB-MP381927PZG
CSB-EP381927PZG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Ribonuclease P protein component (rnpA) CSB-YP381928PZG
CSB-EP381928PZG
CSB-BP381928PZG
CSB-MP381928PZG
CSB-EP381928PZG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 5'-nucleotidase surE (surE) CSB-YP381929PZG
CSB-EP381929PZG
CSB-BP381929PZG
CSB-MP381929PZG
CSB-EP381929PZG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP381930PZG
CSB-EP381930PZG
CSB-BP381930PZG
CSB-MP381930PZG
CSB-EP381930PZG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus D-alanine--D-alanine ligase (ddl) CSB-YP381931PZG
CSB-EP381931PZG
CSB-BP381931PZG
CSB-MP381931PZG
CSB-EP381931PZG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP381932PZG
CSB-EP381932PZG
CSB-BP381932PZG
CSB-MP381932PZG
CSB-EP381932PZG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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