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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Aspergillus niger Riboflavin kinase (fmn1) CSB-YP382115AVE
CSB-EP382115AVE
CSB-BP382115AVE
CSB-MP382115AVE
CSB-EP382115AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Mannitol 2-dehydrogenase (An03g02430) CSB-YP382116AVE
CSB-EP382116AVE
CSB-BP382116AVE
CSB-MP382116AVE
CSB-EP382116AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Putative lipase atg15 (atg15), partial CSB-YP382117AVE
CSB-EP382117AVE
CSB-BP382117AVE
CSB-MP382117AVE
CSB-EP382117AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Arginine biosynthesis bifunctional protein ArgJ, mitochondrial (An03g04330), partial CSB-YP382118AVE
CSB-EP382118AVE
CSB-BP382118AVE
CSB-MP382118AVE
CSB-EP382118AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Probable exopolygalacturonase B (pgxB) CSB-YP382119AVE
CSB-EP382119AVE
CSB-BP382119AVE
CSB-MP382119AVE
CSB-EP382119AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Translation machinery-associated protein 22 (tma22) CSB-YP382120AVE
CSB-EP382120AVE
CSB-BP382120AVE
CSB-MP382120AVE
CSB-EP382120AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Protein transport protein sec13 (sec13) CSB-YP382121AVE
CSB-EP382121AVE
CSB-BP382121AVE
CSB-MP382121AVE
CSB-EP382121AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Cytokinesis protein sepH (sepH), partial CSB-YP382122AVE
CSB-EP382122AVE
CSB-BP382122AVE
CSB-MP382122AVE
CSB-EP382122AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Restriction of telomere capping protein 5 (rtc5), partial CSB-YP382123AVE
CSB-EP382123AVE
CSB-BP382123AVE
CSB-MP382123AVE
CSB-EP382123AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Altered inheritance of mitochondria protein 31, mitochondrial (aim31), partial CSB-YP382124AVE1
CSB-EP382124AVE1
CSB-BP382124AVE1
CSB-MP382124AVE1
CSB-EP382124AVE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Transcriptional activator of proteases prtT (prtT), partial CSB-YP382125AVE
CSB-EP382125AVE
CSB-BP382125AVE
CSB-MP382125AVE
CSB-EP382125AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger ATPase synthesis protein 25, mitochondrial (atp25), partial CSB-YP382126AVE
CSB-EP382126AVE
CSB-BP382126AVE
CSB-MP382126AVE
CSB-EP382126AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Probable endo-xylogalacturonan hydrolase A (xghA) CSB-YP382127AVE
CSB-EP382127AVE
CSB-BP382127AVE
CSB-MP382127AVE
CSB-EP382127AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Probable beta-galactosidase C (lacC), partial CSB-YP382128AVE
CSB-EP382128AVE
CSB-BP382128AVE
CSB-MP382128AVE
CSB-EP382128AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Probable D-xylulose kinase A (xkiA), partial CSB-YP382129AVE
CSB-EP382129AVE
CSB-BP382129AVE
CSB-MP382129AVE
CSB-EP382129AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Mitogen-activated protein kinase mpkC (mpkC) CSB-YP382130AVE
CSB-EP382130AVE
CSB-BP382130AVE
CSB-MP382130AVE
CSB-EP382130AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Putative glucan endo-1,3-beta-glucosidase btgC (btgC), partial CSB-YP382131AVE
CSB-EP382131AVE
CSB-BP382131AVE
CSB-MP382131AVE
CSB-EP382131AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Nuclear distribution protein nudF (nudF) CSB-YP382132AVE
CSB-EP382132AVE
CSB-BP382132AVE
CSB-MP382132AVE
CSB-EP382132AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Probable 1,4-beta-D-glucan cellobiohydrolase A (cbhA) CSB-YP382133AVE
CSB-EP382133AVE
CSB-BP382133AVE
CSB-MP382133AVE
CSB-EP382133AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger WD repeat-containing protein jip5 (jip5) CSB-YP382134AVE
CSB-EP382134AVE
CSB-BP382134AVE
CSB-MP382134AVE
CSB-EP382134AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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