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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Burkholderia pseudomallei 50S ribosomal protein L35 (rpmI) CSB-YP385637BPU
CSB-EP385637BPU
CSB-BP385637BPU
CSB-MP385637BPU
CSB-EP385637BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Ribosome-binding factor A (rbfA) CSB-YP385638BPU
CSB-EP385638BPU
CSB-BP385638BPU
CSB-MP385638BPU
CSB-EP385638BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Leucyl/phenylalanyl-tRNA--protein transferase (aat) CSB-YP385639BPU
CSB-EP385639BPU
CSB-BP385639BPU
CSB-MP385639BPU
CSB-EP385639BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Protein hfq (hfq) CSB-YP385640BPU
CSB-EP385640BPU
CSB-BP385640BPU
CSB-MP385640BPU
CSB-EP385640BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei 30S ribosomal protein S18 (rpsR) CSB-YP385641BPU
CSB-EP385641BPU
CSB-BP385641BPU
CSB-MP385641BPU
CSB-EP385641BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP385642BPU
CSB-EP385642BPU
CSB-BP385642BPU
CSB-MP385642BPU
CSB-EP385642BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP385643BPU
CSB-EP385643BPU
CSB-BP385643BPU
CSB-MP385643BPU
CSB-EP385643BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr) CSB-YP385644BPU
CSB-EP385644BPU
CSB-BP385644BPU
CSB-MP385644BPU
CSB-EP385644BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei DNA ligase (ligA), partial CSB-YP385645BPU
CSB-EP385645BPU
CSB-BP385645BPU
CSB-MP385645BPU
CSB-EP385645BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP385646BPU
CSB-EP385646BPU
CSB-BP385646BPU
CSB-MP385646BPU
CSB-EP385646BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Enolase (eno) CSB-YP385647BPU
CSB-EP385647BPU
CSB-BP385647BPU
CSB-MP385647BPU
CSB-EP385647BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Co-chaperone protein hscB homolog (hscB) CSB-YP385648BPU
CSB-EP385648BPU
CSB-BP385648BPU
CSB-MP385648BPU
CSB-EP385648BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei N-succinylarginine dihydrolase (astB) CSB-YP385649BPU
CSB-EP385649BPU
CSB-BP385649BPU
CSB-MP385649BPU
CSB-EP385649BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei 3-oxoacyl-[acyl-carrier-protein] synthase 3 (fabH) CSB-YP385650BPU
CSB-EP385650BPU
CSB-BP385650BPU
CSB-MP385650BPU
CSB-EP385650BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei 30S ribosomal protein S16 (rpsP) CSB-YP385651BPU
CSB-EP385651BPU
CSB-BP385651BPU
CSB-MP385651BPU
CSB-EP385651BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Integration host factor subunit beta (ihfB) CSB-YP385652BPU
CSB-EP385652BPU
CSB-BP385652BPU
CSB-MP385652BPU
CSB-EP385652BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Ribonuclease PH (rph) CSB-YP385653BPU
CSB-EP385653BPU
CSB-BP385653BPU
CSB-MP385653BPU
CSB-EP385653BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Probable septum site-determining protein MinC (minC) CSB-YP385654BPU
CSB-EP385654BPU
CSB-BP385654BPU
CSB-MP385654BPU
CSB-EP385654BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei N utilization substance protein B homolog (nusB) CSB-YP385655BPU
CSB-EP385655BPU
CSB-BP385655BPU
CSB-MP385655BPU
CSB-EP385655BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Urease subunit alpha (ureC), partial CSB-YP385656BPU
CSB-EP385656BPU
CSB-BP385656BPU
CSB-MP385656BPU
CSB-EP385656BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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