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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Burkholderia pseudomallei UPF0301 protein BURPS668_3112 (BURPS668_3112) CSB-YP385657BPU
CSB-EP385657BPU
CSB-BP385657BPU
CSB-MP385657BPU
CSB-EP385657BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Chaperone protein DnaJ (dnaJ) CSB-YP385658BPU
CSB-EP385658BPU
CSB-BP385658BPU
CSB-MP385658BPU
CSB-EP385658BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Heat-inducible transcription repressor HrcA (hrcA) CSB-YP385659BPU
CSB-EP385659BPU
CSB-BP385659BPU
CSB-MP385659BPU
CSB-EP385659BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP385660BPU
CSB-EP385660BPU
CSB-BP385660BPU
CSB-MP385660BPU
CSB-EP385660BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Putative iron-sulfur cluster insertion protein ErpA (erpA) CSB-YP385661BPU
CSB-EP385661BPU
CSB-BP385661BPU
CSB-MP385661BPU
CSB-EP385661BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Leucine--tRNA ligase (leuS), partial CSB-YP385662BPU
CSB-EP385662BPU
CSB-BP385662BPU
CSB-MP385662BPU
CSB-EP385662BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Cyanate hydratase (cynS) CSB-YP385663BPU
CSB-EP385663BPU
CSB-BP385663BPU
CSB-MP385663BPU
CSB-EP385663BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Proline--tRNA ligase (proS), partial CSB-YP385664BPU
CSB-EP385664BPU
CSB-BP385664BPU
CSB-MP385664BPU
CSB-EP385664BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei 50S ribosomal protein L21 (rplU) CSB-YP385665BPU
CSB-EP385665BPU
CSB-BP385665BPU
CSB-MP385665BPU
CSB-EP385665BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP385666BPU
CSB-EP385666BPU
CSB-BP385666BPU
CSB-MP385666BPU
CSB-EP385666BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP385667BPU
CSB-EP385667BPU
CSB-BP385667BPU
CSB-MP385667BPU
CSB-EP385667BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Indole-3-glycerol phosphate synthase (trpC) CSB-YP385668BPU
CSB-EP385668BPU
CSB-BP385668BPU
CSB-MP385668BPU
CSB-EP385668BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP385669BPU
CSB-EP385669BPU
CSB-BP385669BPU
CSB-MP385669BPU
CSB-EP385669BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei Protein CyaY (cyaY) CSB-YP385670BPU
CSB-EP385670BPU
CSB-BP385670BPU
CSB-MP385670BPU
CSB-EP385670BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei 30S ribosomal protein S11 (rpsK) CSB-YP385671BPU
CSB-EP385671BPU
CSB-BP385671BPU
CSB-MP385671BPU
CSB-EP385671BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei 50S ribosomal protein L15 (rplO) CSB-YP385672BPU
CSB-EP385672BPU
CSB-BP385672BPU
CSB-MP385672BPU
CSB-EP385672BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei 30S ribosomal protein S8 (rpsH) CSB-YP385673BPU
CSB-EP385673BPU
CSB-BP385673BPU
CSB-MP385673BPU
CSB-EP385673BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei 50S ribosomal protein L24 (rplX) CSB-YP385674BPU
CSB-EP385674BPU
CSB-BP385674BPU
CSB-MP385674BPU
CSB-EP385674BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei 50S ribosomal protein L29 (rpmC) CSB-YP385675BPU
CSB-EP385675BPU
CSB-BP385675BPU
CSB-MP385675BPU
CSB-EP385675BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei 50S ribosomal protein L22 (rplV) CSB-YP385676BPU
CSB-EP385676BPU
CSB-BP385676BPU
CSB-MP385676BPU
CSB-EP385676BPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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